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目的建立HepG2细胞胰岛素抵抗模型,并用于评价滇越金线兰中2个丁酸衍生物对胰岛素敏感性的改善作用。方法采用高糖高浓度胰岛素诱导HepG2细胞建立肝细胞胰岛素抵抗模型,研究滇越金线兰2个丁酸衍生物对胰岛素抵抗模型细胞葡萄糖摄取和细胞活力的影响。结果胰岛素浓度为10-7mol·L-1时细胞的葡萄糖摄取量最小,为合适的模型复制浓度。与模型组比较,2个丁酸衍生物都能显著增加胰岛素抵抗的HepG2细胞对葡萄糖的吸收,葡萄糖摄取量分别增加了76.7豫和77.8豫,半数有效浓度(EC50)分别为9.372和10.854滋mol·L-1,对胰岛素抵抗的HepG2细胞的葡萄糖摄取呈现较好的促进作用。结论 2个丁酸衍生物对胰岛素具有增敏活性,能有效促进胰岛素抵抗细胞对葡萄糖的摄取和利用。
Objective To establish a model of insulin resistance in HepG2 cells and to evaluate the effects of two butyric acid derivatives on the insulin sensitivity in the extract of HepG2. Methods HepG2 cells were induced by high glucose and high concentration of insulin to establish hepatocyte insulin resistance model and to study the effect of 2 butyric acid derivatives of Jinnweilai on the glucose uptake and cell viability of insulin resistance model. Results When the insulin concentration was 10-7mol·L-1, the glucose uptake of the cells was the smallest, which was the appropriate model replication concentration. Compared with the model group, 2 butyric acid derivatives can significantly increase the glucose uptake in HepG2 cells with insulin resistance, the glucose uptake increased by 76.7 and 77.8, respectively, and the EC50 values were 9.372 and 10.854 mmol · L-1, glucose uptake of insulin-resistant HepG2 cells showed a better role in promoting. Conclusion 2 butyric acid derivatives have sensitizing activity to insulin and can effectively promote glucose uptake and utilization by insulin resistant cells.