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目的:探讨冠心病患者阿司匹林抵抗(AR)与CD4+ CD25+调节性T细胞(Treg)的关系.方法:选取112例冠心病AR患者为观察组(AR组),冠心病阿司匹林敏感(AS)患者(AS组)及健康对照组各112例为对照组,使用流式细胞术检测各组外周血Treg及其细胞因子转化生长因子β1(TGF-β1)及白细胞介素10(IL-10)的百分含量;使用花生四烯酸(AA)及二磷酸腺苷(ADP)作为诱导剂测定血小板聚集率(PAR).将冠心病AR患者随机分为氯吡格雷及复方丹参滴丸两组分别进行干预,比较上述指标的变化.结果:AR组及AS组Treg、TGF-β1及IL-10均显著降低,且显著低于健康对照组(P＜0.05),AR组明显低于AS组(P＜0.05).AR及AS组AA-PAR及FDP PAR均显著高于健康对照组,3组间两两比较差异均有统计学意义(均P＜0.05).治疗后氯吡格雷组Treg、TGF-β1及IL-10均显著高于复方丹参滴丸组(P＜0.05),且AA-PAR及FDP-PAR显著低于复方丹参滴丸组(P＜0.05).相关性分析显示,Treg、TGF-β1及IL 10均与AA-PAR及FDP-PAR呈显著负相关性.结论:冠心病AR患者Treg、TGF-β1及IL-10均明显降低,其与PAR呈负相关性,提示Treg可能是AR患者的保护性因素.“,”Objective:To investigate the correlation between aspirin resistance (AR) and regulatory T cells in patients with coronary heart disease (CHD).Method:One hundred and twelve CHR patients with AR were included into observation group (AR group),while 112 cases CHD who patients were sensitive to aspirin (AS group)and 112 healthy controls (healthy control group)were included as control groups.Flow cytometry was used to test peripheral blood Treg growth factors and cytokines 1 (TGF-β1) and interleukin 10 (IL-10) percentage.Arachidonic acid (AA) and adenosine diphosphate (ADP) were used to determine platelet aggregation rate (PAR).AR patients were randomly divided into clopidogrel group and compound danshen dropping pill group,then the indicators above were compared.Result:The levels of Treg,TGF-β1 and IL-10 were significantly lower in patients with AR and AS than in healthy controls (all P＜0.05),but those were lower in AR group than in AS group (all P＜0.05).AA-PAR and FDP-PAR were significantly higher in patients with AR and AS than in healthy controls (all P＜0.05),and the differences among the three groups were statistical (all P＜0.05).After treatment,the levels of Treg,TGF β1 and IL-10 were significantly higher,while AA-PAR and FDP-PAR significantly lower in clopidogrel group than those in compound danshen dropping pill group (all P＜0.05).Correlation analysis showed that Treg,TGF-β1 and IL-10 were negative correlation with AA-PAR and FDP-PAR.Conclusion:The levels of Treg,TGF-β1 and IL-10 are significantly lower,and negative correlation with PAR in CHD patients with AR.Treg may be protective factors for patients with AR.