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群众杨插条苗经 PEG渗透胁迫处理后 ,取根部样品 ,经过快速冷冻、冷冻干燥、乙醚真空渗透及塑料包埋后 ,用透射电镜 X-射线能谱微区分析技术 ,对根表皮层、皮层及中柱薄壁组织细胞的细胞壁、细胞质和液泡中的 K+含量与分布进行了测定。结果表明 ,在渗透胁迫下 ,在根各部位组织细胞的细胞壁、细胞质和液泡中 ,K+含量都有不同程度的增加 ,增加的 K+主要积累在液泡中。渗透胁迫所促进的 K+吸收可被蛋白质合成抑制剂环己酰亚胺所抑制。
After treated with PEG osmotic stress, the Populus trumpet popularize seedlings were taken from the root samples and subjected to rapid freezing, freeze-drying, vacuum infiltration of diethyl ether and plastic embedding, and then analyzed by transmission electron microscopy X-ray energy spectrum microanalysis to analyze the root epidermis, The content and distribution of K + in the cell wall, cytoplasm and vacuole of cortical and medial column parenchyma cells were determined. The results showed that under osmotic stress, the content of K + increased to some extent in the cell wall, cytoplasm and vacuole of the tissue cells in all parts of the root, and the increased K + mainly accumulated in the vacuole. The K + uptake promoted by osmotic stress can be inhibited by the cycloheximide, a protein synthesis inhibitor.