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目的研究安佳欣胶囊对抑郁症模型大鼠基因功能类表达的影响。方法分析抑郁症大鼠模型组和安佳欣胶囊组各8例样本的基因表达谱数据,筛选差异表达基因。利用基因功能分类体系Gene Ontology中的生物学过程子树和细胞组分子树,寻找和分析显著聚集差异表达基因的复合功能类,从分子水平和基因功能模块水平探索安佳欣胶囊对抑郁症模型大鼠基因表达谱的影响。结果筛选出330个差异表达基因,并进一步识别了8个差异表达基因功能模块,主要涉及糖代谢、蛋白转运、谷氨酰胺代谢、凋亡诱导和神经发生。通过文献证实进一步发现了差异功能模块中7个可能与抑郁症发病相关的基因(Pfkm、Gpx1、Stx1a、Ninj2、Plp、Evl、Nrn1)在两组动物中差异表达。结论安佳欣胶囊可能增加抑郁症模型大鼠的单胺递质合成和释放,改善神经保护和神经发生功能,这些改变可能与安佳欣胶囊的抗抑郁作用有关。
Objective To study the effect of Anjiaxin capsule on the expression of gene functional class in rat model of depression. Methods The gene expression profiles of eight samples from each group of depression model rats and one from Anjiaxin capsule group were analyzed. Differentially expressed genes were screened. Using the biological process sub-trees and cell group molecular trees in Gene Ontology, to find and analyze complex functional groups that significantly cluster differentially expressed genes, and to explore Anjiaxin capsules for depression models at the molecular level and at the level of gene function modules The effect of gene expression profiles in rats. The results screened 330 differentially expressed genes and further identified 8 differentially expressed gene functional modules, mainly involving glucose metabolism, protein transport, glutamine metabolism, apoptosis induction and neurogenesis. Through literature verification, it was further found that seven genes (Pfkm, Gpx1, Stx1a, Ninj2, Plp, Evl, Nrn1) that may be involved in the development of depression were differentially expressed in two functional groups. Conclusion Anjiaxin capsule may increase the synthesis and release of monoamine neurotransmitters in rats with depression, and improve neuroprotection and neurogenesis. These changes may be related to the antidepressant effects of Anjiaxin capsules.