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采用体外细胞培养技术,观察了钙离子阻断剂3、6-[=甲氨基]-=苯骈碘杂六环枸橼酸盐(I-65)单独和与柔红霉素(DNR)合用对L_(1210)细胞增殖的影响。结果:单独应用I-65时对细胞的增殖和集落形成抑制作用极小;与DNR合用,可显著增强DNR对细胞增殖的抑制作用。其ID_(50)为单用DNR的1/11。联合用药时细胞集落形成率较单用DNR(0.15μg/ml)下降14倍,证实I-65能显著增强DNR的抗癌活性,为广泛应用DNR,减轻心脏毒性,防止耐药提供新手段,为临床提供了一种新的抗癌药物增效剂。
The in vitro cell culture technique was used to observe the effect of calcium ion blockers 3,6 - [= methylamino] - = benzodiazepine citrate (I-65) alone and in combination with daunorubicin (DNR) On the proliferation of L_ (1210) cells. RESULTS: I-65 alone had minimal inhibitory effect on cell proliferation and colony formation. Combined with DNR, it could significantly enhance the inhibitory effect of DNR on cell proliferation. Its ID_ (50) is 1/11 of DNR alone. Compared with DNR (0.15μg / ml) alone, the rate of colony formation decreased by 14-fold. The results showed that I-65 could significantly enhance the anti-cancer activity of DNR and provide a new means to widely use DNR, reduce cardiotoxicity and prevent drug resistance. Provides a new anticancer drug synergist for the clinic.