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介绍了分子标记的4 个系统:①非PCR 标记系统;②以RAPD 为代表的单引物PCR 系统;③由两个引物引起的选择性扩增片段多态性(AFLP) ;④以SSR( 或microsatellite) 为代表的两个引物的PCR 系统,以及它们各自的特点。同时还提出了在具体应用时应从5 个方面考虑、选择合适的标记系统。
Four systems of molecular markers were introduced: ① non-PCR labeling system; ② single-primer PCR system represented by RAPD; ③ selective amplified fragment polymorphism (AFLP) caused by two primers; ④ SSR microsatellite) as the representative of the two primers PCR system, as well as their respective characteristics. At the same time, it also puts forward that in the specific application, we should consider from five aspects and choose the appropriate marking system.