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采用室内试验和田间试验相结合方式,研究了不同浓度(0、0.01、0.05、0.10、0.50和1.00mmol·L-1)2,4-二叔丁基苯酚对番茄叶霉菌及番茄种子萌发幼苗生长的影响。结果表明:2,4-二叔丁基苯酚在叶霉菌培养的前阶段极显著地抑制了菌丝的生长,培养7d时,0.1mmol·L-1的抑制作用最大,抑制率为40%;在田间抗病性调查期间各浓度处理均提高了番茄幼苗的抗病性,其中以0.1mmol·L-1处理抗病性最好,病情指数比对照降低了54%;低浓度2,4-二叔丁基苯酚促进了番茄种子的萌发,增加了幼苗的株高、茎粗、地上和地下部鲜质量、叶绿素含量、根系活力和幼苗体内保护酶活性,随着浓度增大,促进作用减弱甚至表现出抑制作用;而低浓度的2,4-二叔丁基苯酚降低了叶片丙二醛(MDA)含量和细胞相对电导率,且随浓度增加作用强度增大;浓度为0.1mmol·L-1时,抑制作用最强。综合分析表明,2,4-二叔丁基苯酚对番茄植株及叶霉病具有调控作用,可以确定0.1mmol·L-1为田间施加最适浓度。
The effects of 2,4-di-tert-butylphenol with different concentrations (0,0.01,0.05,0.10,0.50 and 1.00mmol·L-1) on seed germination of tomato leaf mold and tomato seedlings were studied by the combination of indoor and field experiments. The impact of growth. The results showed that 2,4-di-tert-butylphenol inhibited the mycelium growth significantly at the pre-stage of leaf mold culture. The inhibition rate of 0.1mmol·L-1 was the highest at the 7th day after culture, with the inhibition rate of 40%. The disease resistance of tomato seedlings was increased by each concentration treatment during the field disease resistance investigation. Among them, the disease resistance was the best when treated with 0.1 mmol·L-1, and the disease index was reduced by 54% compared with the control. Low concentration of 2,4- Di-tert-butylphenol promoted the germination of tomato seeds, increased seedling height, stem diameter, fresh weight above and below the ground, chlorophyll content, root vigor and protective enzyme activities in seedlings. As the concentration increased, the promotion effect was weakened Even at low concentration of 2,4-di-tert-butylphenol reduced MDA content and cell relative conductivity, and increased with increasing concentration of strength; concentration of 0.1mmol · L -1, the inhibition is strongest. The comprehensive analysis showed that 2,4-di-tert-butylphenol had a regulatory effect on tomato plants and leaf mold, and 0.1 mmol·L-1 was the optimal concentration for field application.