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从苹果金冠基因组中鉴定得到18个生长素输出载体蛋白PIN-formed(PIN),对其进行理化特性、基因结构、系统进化和启动子元件分析。结果表明,MdPIN基因家族中,含有1~14个外显子,0~13个内含子;MdPIN蛋白的保守基序数量在3~10。苹果PIN和拟南芥PIN等蛋白高度同源,且根据其进化树分为G1、G2和G3亚组;18个MdPIN在不同基因型苹果的不同器官中表达具有明显差异。以‘长富2号’为材料,从其腋芽中克隆得到一个候选基因MdPIN15,其开放阅读框为1869 bp,编码622个氨基酸。实时定量PCR表明,MdPIN15在梢尖部位表达量最高,其次是腋芽,在花芽中表达量最低;,外源GR24和Lovastatin(LVS)处理降低MdPIN15的表达量;6-BA和去茎尖处理提高了MdPIN15的表达量。MdPIN15在介导细胞分裂素(CK)、生长素(IAA)和独脚金内酯(SL)等激素调控腋芽萌发有重要作用。
Eighteen auxin-exporting vector PIN-formed (PIN) proteins were identified from apple golden crown genome, and their physical and chemical properties, gene structure, phylogenetic analysis and promoter element analysis were analyzed. The results showed that the MdPIN gene family contains 1 ~ 14 exons and 0 ~ 13 introns. The number of conserved motifs in MdPIN protein ranged from 3 to 10. The apple PIN and Arabidopsis PIN proteins were highly homologous and classified into G1, G2 and G3 subgroups according to their phylogenetic tree. The 18 MdPINs were significantly different in different organs of different genotype apples. A new gene, MdPIN15, was cloned from axillary buds of Chang Fu 2 using an open reading frame of 1869 bp, encoding 622 amino acids. Real-time quantitative PCR showed that MdPIN15 had the highest expression at the shoot tip, followed by the axillary bud and the lowest expression in the flower bud. Exogenous GR24 and Lovastatin (LVS) decreased the expression of MdPIN15; The expression level of MdPIN15. MdPIN15 plays an important role in the regulation of hormonal regulation of axillary buds such as cytokinin (CK), auxin (IAA) and strigolactone (SL).