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本研究选取黄河三角洲地区的盐碱土,将高盐和低盐土按照不同比例配制成试验用土,在水稻两叶一心期,测定供试12个水稻材料的9个与耐盐相关的形态学和生理学指标,并对其苗期耐盐指标进行筛选。结果表明,在0.34%盐浓度下,水稻苗期的株高、干物质积累等受到抑制,不同材料之间的叶绿素含量、丙二醛含量和抗氧化酶活性差异明显。对各性状测定数据为参数的耐盐指数进行主成分分析,提取出两个综合指标,贡献率分别为55.588%和24.054%,累积贡献率为79.642%。根据D值得出海稻86对盐的耐受性最强,其次为盐粳218、日本晴。通过逐步回归分析建立的最优回归方程表明,幼苗鲜重、叶绿素b含量、超氧化物歧化酶活性、丙二醛含量和株高这5项指标对水稻苗期的耐盐性有显著影响,可作为该地区水稻苗期的耐盐鉴定指标。
In this study, we selected saline-alkali soil in the Yellow River Delta region, and formulated high-salt and low-salt soil into test soil according to different proportions. During the two-leaf and one-leaf period of rice, nine of the 12 rice materials tested were salt-tolerant and their morphology and physiology Indicators, and salt tolerance indicators of its seedling screening. The results showed that at 0.34% salt concentration, plant height and dry matter accumulation in rice seedling stage were inhibited. Chlorophyll content, malondialdehyde content and antioxidant enzyme activities differed significantly among the different materials. Principal component analysis (PCA) of salt tolerance index of each trait was used as parameter, and two comprehensive indexes were extracted. The contribution rate was 55.588% and 24.054% respectively, and the cumulative contribution rate was 79.642%. According to the value of D, sea salt 86 was the most tolerant to salt, followed by salt japonica 218 and Nipponbare. The regression equation established by stepwise regression analysis showed that fresh weight, chlorophyll b content, superoxide dismutase activity, malondialdehyde content and plant height of seedlings had significant effects on salt tolerance of rice at seedling stage, It can be used as salt tolerance index in rice seedling stage in this area.