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疫霉菌分泌的RxLR效应子能干扰寄主植物细胞的正常生理代谢和功能。我们通过同源序列搜索在辣椒疫霉菌中鉴定到9个PiAvr3a的同源基因(PiAvr3a-like),同时发现PiAvr3a-like在大豆疫霉菌中也有分布,表明该类基因可能在疫霉菌致病过程中发挥重要作用。从辣椒疫霉菌中成功克隆了5个PiAvr3a-like基因,表达模式分析发现PcAvh128/132/134这3个基因在疫霉菌侵染早期诱导表达,暗示它们可能在侵染前期发挥功能。接种实验表明在本氏烟中瞬时表达其中的PcAvh128可以显著促进辣椒疫霉菌的侵染,而PcAvh132活性相反,其他3个基因没有明显活性。这5个基因均不能抑制INF1和效应因子诱导的细胞死亡,也不能诱导可见的过敏反应。上述结果表明PiAvr3a-like基因不同成员之间功能存在分化,这为疫霉菌和寄主的分子互作研究提供了材料和参考。
RxLR effectors secreted by Phytophthora can interfere with the normal physiological metabolism and function of host plant cells. We identified 9 PiAvr3a-like genes in Phytophthora capsici by homologous sequence search, and also found that PiAvr3a-like was also found in Phytophthora sojae, indicating that these genes may be involved in the pathogenesis of Phytophthora capsici In the play an important role. Five PiAvr3a-like genes were successfully cloned from Phytophthora capsici. Expression pattern analysis showed that the PcAvh128 / 132/134 genes were induced in the early stage of Phytophthora infestans, indicating that they may function in the early stage of infection. Inoculation experiments showed that transient expression of PcAvh128 in N. benthamiana could significantly promote the infection of P. capsici, while the activity of PcAvh132 was opposite. The other three genes had no obvious activity. None of these five genes inhibited INF1 and effector-induced cell death or induced a visible allergic reaction. The above results indicate that the functional differentiation of different members of PiAvr3a-like gene provides material and reference for the interaction of Phytophthora and host.