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目的：克隆和鉴定人淋巴细胞免疫反应性生长激素（ｉｒＧＨ）基因调控序列的核苷酸顺序。方法：通过ＰＣＲ扩增出人淋巴细胞ｉｒＧＨ基因５′近端的调控序列，然后将其与质粒ＰＧＥＭ７Ｚｆ（＋）连接，经转化蓝白斑筛选、酶切鉴定，获得ＰＧＥＭ７Ｚｆ（＋）ｉｒＧＨＤＮＡ（－４８４＋２ｂｐ）重组质粒，进行序列测定。结果：显示ｉｒＧＨ基因５′近端的调控序列与垂体细胞ＧＨ基因的５′近端调控序列的核苷酸顺序完全相同。结论：该结果进一步证明免疫系统与神经内分泌系统的密切关系
OBJECTIVE: To clone and identify the nucleotide sequence of regulatory sequences of human lymphocyte immune-responsive growth hormone (irGH) gene. Methods: The 5 ’proximal regulatory sequence of irGH gene was amplified by PCR, then ligated with PGEM7Zf (+), transformed into blue and white plaques and identified by restriction enzyme digestion. PGEM7Zf (+) irGHDNA (- 484 + 2bp) recombinant plasmid for sequence determination. Results: The nucleotide sequence of the 5 ’proximal regulatory sequence of the irGH gene is identical to the nucleotide sequence of the 5’ proximal regulatory sequence of the pituitary GH gene. Conclusion: This result further demonstrates the close relationship between immune system and neuroendocrine system