论文部分内容阅读
目的··:观察大鼠吗啡依赖和戒断状态下脑内前阿黑皮素原 (POMC )mRNA的变化。方法·· :采用腹腔注射法 ,按剂量递增原则建立大鼠吗啡依赖动物模型 ,同时建立生理盐水对照组。吗啡依赖大鼠随机分为依赖组和戒断组。对照组和依赖组动物于末次注射后3h ,戒断组大鼠于末次注射后72h迅速断头处死取脑 ,收集各个脑区组织总RNA标本。以POMC反义RNA探针对得到的RNA标本进行NorthernBlot印迹杂交 ,用电子计算机图像处理系统对所得结果进行分析。结果·· :吗啡依赖大鼠下丘脑POMCmRNA的表达仅为生理盐水对照组的69.56%±s4.7 % ,戒断72h状态下的吗啡依赖大鼠下丘脑POMCmRNA的表达恢复至生理盐水对照组的78.26 %±s5.3 % ;海马、纹状体和垂体POMCmRNA表达均为阴性。结论··:吗啡依赖大鼠下丘脑POMCmRNA的表达较生理盐水对照组有显著下降 ,戒断72h状态下的吗啡依赖大鼠其下丘脑POMCmRNA的表达较依赖组大鼠有显著回升 ,但仍显著低于正常组水平。此结果从分子生物学水平证实阿片类药物依赖机制与其引起内源性阿片肽系统功能变化有关
Objective · · To observe the changes of premesogenic melanocortin (POMC) mRNA in morphine-dependent and withdrawal rats. Methods · · ·: intraperitoneal injection, according to the principle of increasing dose of morphine-dependent rat model, while the establishment of saline control group. Morphine-dependent rats were randomly divided into dependent group and withdrawal group. At 3h after the last injection, the rats in the control group and the dependent group were sacrificed by decapitation 72h after the last injection, and the total RNA samples of each brain region were collected. The obtained RNA samples were subjected to NorthernBlot blotting with POMC antisense RNA probe, and the results were analyzed by an electronic computer image processing system. RESULTS: The expression of POMC mRNA in the hypothalamus of morphine dependent rats was only 69.56% ± s4.7% of the saline control group. The expression of POMC mRNA in the hypothalamus of morphine dependent rats returned to normal saline control group 78.26% ± s5.3%. The expression of POMC mRNA in hippocampus, striatum and pituitary was negative. Conclusions · · · The morphine-dependent rats hypothalamus POMC mRNA expression decreased significantly compared with the saline control group, morphine-dependent rats at 72h withdrawal hypothalamus POMC mRNA expression than the dependent group rats significantly increased, but still significant Lower than the normal group level. This result confirms from the molecular biology level that opioid dependence mechanisms are related to changes in the function of endogenous opioid peptides