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目的研究生脉注射液活性成分人参皂苷Rg1、人参皂苷Re、五味子醇甲在正常和心肌缺血状态下Beagle犬体内的药动学变化。方法选用Agilent ZORBAX SB-C18色谱柱,流动相为乙腈-水,梯度洗脱,质谱采用选择反应监测(SIM)方式进行正离子检测3种活性成分。用Win Nonlin 6.3软件和SPSS 19.0软件计算各给药组药动学参数,并进行统计分析。结果造模后,人参皂苷Rg1半衰期缩短;人参皂苷Re表观分布容积增大,肾清除率减慢;模型组五味子醇甲半衰期延长,体内平均滞留时间延长,表观分布容积增大,肾清除率减慢。结论 Beagle犬滴注生脉注射液后,人参皂苷Rg1、人参皂苷Re、五味子醇甲在心肌缺血模型犬体内的药动学特征,与正常犬的药动学特征存在很大差异。
Objective To study the pharmacokinetics of Shengmai injection active ingredient ginsenoside Rg1, ginsenoside Re, Schizandrin A in Beagle dogs under normal and myocardial ischemia. Methods The Agilent ZORBAX SB-C18 column was used. The mobile phase consisted of acetonitrile-water and gradient elution. The mass spectra were monitored by selective reaction monitoring (SIM). The pharmacokinetic parameters of each administration group were calculated by Win Nonlin 6.3 software and SPSS 19.0 software, and statistical analysis was performed. Results After modeling, the half-life of ginsenoside Rg1 was shortened; the apparent volume of distribution of ginsenoside Re was increased and the rate of renal clearance was slowed down. The half-life of schizandrin in model group was prolonged, the mean residence time in the body was prolonged, the apparent volume of distribution was increased, Slow down. Conclusion The pharmacokinetics of ginsenoside Rg1, ginsenoside Re and Schizandrin A in dogs with myocardial ischemia were significantly different from those of normal dogs after Beagle dogs were injected Shengmai injection.