论文部分内容阅读
目的制备低分子肝素聚乳酸-羟基乙酸(LWMH-PLGA)缓释微球,观察微球表面形态,检测微球物理性能和体外释药行为。方法采用W1/O/W2复乳溶剂挥发法制作微球;通过扫描电镜观察微球的表面形态结构;利用天青A比色法测试微球中药物的载药量和包封率,并对微球中药物的体外释放行为进行研究。结果微球表面显现较多的孔隙,平均粒径为(2.55±0.94)μm,载药量为(14.79±1.03)%,包封率为(55.7±2.21)%;48 h的体外释放试验表明,LWMH累积释放率达到40%。结论 LWMH-PLGA微球能够稳定地释放药物LWMH,验证了PLGA微球作为LWMH控制释放载体的可行性。
Objective To prepare LWMH-PLGA sustained-release microspheres, observe the morphology of the microspheres and determine their physical properties and in vitro release behavior. Methods The microspheres were prepared by W1 / O / W2 complex emulsion solvent evaporation method. The morphology of the microspheres was observed by scanning electron microscopy. The drug loading and entrapment efficiency of the microspheres were measured by Azure A colorimetric assay. The in vitro drug release behavior of the microspheres was studied. Results The microspheres showed more pores on the surface, the mean diameter was (2.55 ± 0.94) μm, the drug loading was (14.79 ± 1.03)% and the entrapment efficiency was (55.7 ± 2.21)%. , LWMH cumulative release rate of 40%. Conclusion LWMH-PLGA microspheres can release drug LWMH stably and demonstrate the feasibility of PLGA microspheres as LWMH controlled release vector.