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建立了一种在表面活性剂OP存在下 ,用 2 - (5-溴 - 2 -吡啶偶氮 ) - 5-二乙氨基酚(5-Br-PADAP)作显色剂分光光度法同时测定铜和铁含量的新方法。用该法测定铜和铁 ,显色络合物最大吸收波长均为 558nm ;pH 3 5时 ,测得Cu - 5-Br -PADAP的ε为 9 1× 10 4L·mol- 1cm- 1,Fe- 5-Br -PADAP的ε为 7 2× 10 4 L·mol- 1·cm- 1;铜线性范围为 0～ 4 0μg/ 10mL,铁线性范围为 0～ 5 0 μg/ 10mL。此法应用于人发样品分析 ,方法简便、快速 ,结果准确、满意。
A novel method for the simultaneous determination of copper (Ⅱ) with copper (Ⅱ) in the presence of surfactant OP by spectrophotometry using 2 - (5 - bromo - 2 - pyridylazo) - 5 - diethylaminophenol And the new method of iron content. The maximum absorption wavelength of the chromogenic complex was 558nm when Cu and Fe were determined by this method. The ε of Cu - 5 - Br - PADAP was 9 1 × 10 4 L · mol -1 cm -1 at pH 3.5, the Fe The ε of 5-Br-PDA was 7 2 × 10 4 L · mol -1 cm -1; the linear range of copper was 0 ~ 40 μg / 10 mL; the linear range of iron was 0 ~ 50 μg / 10 mL. This method is applied to human hair sample analysis, the method is simple, fast, accurate and satisfactory.