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利用来自 87个F2 代单株的 81 0个标记 (784个AFLP标记和 2 6个SSR标记 )构建了美洲黑杨×青杨杂种分子连锁遗传图谱。该图谱包括 1 9个主要连锁群 ,共有 36 8个标记 (35 6个AFLP标记和 1 2个SSR标记 )。框架图总图距为 3382 4cM ,平均标记间距为 9 6 9cM。利用区间作图法在LOD≥ 2 ,检测区间为 2cM条件下检测到 8个与木材性状有关的QTLs。其中与木材基本密度关联的 3个QTLs贡献率分别为 2 7 1 %、2 7 1 %、2 5 5 % ,与微纤丝角关联的QTLs贡献率为 1 8 6 % ,与纤维长度关联的QTLs贡献率为 4 8 9% ,与纤维宽关联的 3个QTLs贡献率分别为 1 7 4 %、2 7 4 %和 4 8 3%。这 8个QTLs遗传作用方式均为超显性 ,其中只有 2个与基本密度关联的QTLs来自于美洲黑杨 ,其余 6个均来自于青杨。
A linkage map was constructed using 81 0 markers (784 AFLP markers and 26 SSR markers) from 87 F2 generation plants. The map consisted of 19 main linkage groups with a total of 368 markers (356 AFLP markers and 12 SSR markers). Frame map with a total map of 3382 4cM, with an average marker spacing of 9 6 9cM. Using interval mapping method, 8 QTLs related to wood traits were detected under the condition of LOD≥2 and the detection interval of 2cM. Among them, the contribution rate of three QTLs correlated with wood basic density was 27.1%, 27.1% and 25.5% respectively, and the contribution rate of QTLs associated with microfibril angle was 186%, which was correlated with fiber length The contribution rate of QTLs was 48.9%, and the contribution rate of three QTLs associated with fiber width was 174%, 274% and 483% respectively. The genetic effects of these eight QTLs were all over dominant. Only two QTLs associated with their basic density were from Populus nigra. The other six were from Populus.