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目的:实验研究铅中毒以阐明血清酶活性逆向变化的机制.方法:Wistar雌性大鼠随机分为4组,对照组,对照组10,30和60mg/kg组.醋酸铅腹腔注射,隔天染毒1次,7次后处死动物,取血分离血清,测血清酶活性.另分别向大鼠血清中加入醋酸铅使其终浓度为0,30及300μmol/L,37℃孵育3h,测定血清酶活性.结果:体内实验表明,随染铅剂量的增加,血清ALP与LDH活性变化呈下降趋势(P<0.05);而γ-GT及AST活性变化呈上升趋势(与对照组比较,P<0.05);ALT活性变化先下降(与对照组比较,P<0.05),而后随铅剂量的增加而呈上升趋势.体外实验表明,铅对ALT活性抑制较明显(与对照组比较,P<0.05),而对LDH,ALP,AST及γ-GT活性未见有抑制作用.结论:铅中毒所引起的部分血清酶活性逆向变化的机制是由于其活性受到铅等因素不同程度的抑制所致.
Objective: To study the mechanism of lead poisoning in order to clarify the reverse change of serum enzyme activity.Methods: Wistar female rats were randomly divided into 4 groups: control group, 10, 30 and 60 mg / kg control group, The animals were sacrificed once, seven times, the blood was taken to separate the serum and the activities of serum enzymes were measured.And lead acetate was added into rat serum to make the final concentrations of 0, 30 and 300μmol / L, incubated for 3h at 37 ℃, Results: In vivo, the changes of serum ALP and LDH activity showed a decreasing trend with the increase of lead dose (P <0.05), while the activity of γ-GT and AST showed an upward trend (P <0.05 compared with the control group) ; ALT activity decreased first (compared with the control group, P <0.05), then increased with the increase of lead dose.Expression of lead in ALT activity was significantly inhibited in vitro (compared with the control group, P <0.05) But no inhibitory effect on the activity of LDH, ALP, AST and γ-GT.Conclusion: The mechanism of the reverse change of some serum enzyme activities caused by lead poisoning is due to the inhibition of lead and other factors to varying degrees.