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采用氧瓶燃烧法对血样进行破坏 ,利用 β_环糊精和十二烷基硫酸钠形成包合物的性质 ,将4,5_苯并苯硒二唑包合 ,建立了三元包合物荧光分析法 ,不经萃取 ,直接测定血浆中的硒。方法的日内精密度为5.4 %～9.3 %(n=7) ;日间精密度为3.5 %～14.5 %(n=7) ;线性范围为10～500μg/L ;回收率为91.0 %～97.8 %。对新疆汉族、维吾尔族和沈阳汉族58人的血样进行测定 ,结果表明 :新疆汉族与沈阳汉族间血硒值无显著性差异(F=1.36 ,P>0.05) ;新疆汉族与维吾尔族间血硒值有非常显著性差异(F=1.01 ,P<0.01) ;男女间血硒值有非常显著性差异(P<0.01)。
Blood samples were destroyed by the oxygen bottle combustion method. By using β_cyclodextrin and sodium lauryl sulfate, the inclusion complex was formed. The inclusion of 4,5-benzofuran selenodiazole Fluorescence analysis method, without extraction, direct determination of plasma selenium. The intra-day precision ranged from 5.4% to 9.3% (n = 7), the intra-day precision ranged from 3.5% to 14.5% (n = 7), the linear range was from 10 to 500 μg / L and the recoveries ranged from 91.0% to 97.8% . The blood samples of 58 Han, Uygur and Han Chinese in Xinjiang were measured. The results showed that there was no significant difference in blood selenium between Xinjiang Han and Shenyang Han (F = 1.36, P> 0.05) (F = 1.01, P <0.01). There was a significant difference in blood selenium between men and women (P <0.01).