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手术取人的正常皮肤,在无血清培养基中添加小柴胡汤提取剂,培养增殖细胞。以酶联免疫吸附(ELISA)测定上清液中IL-lα和IL-6的活性。小柴胡汤毒性用MTT法测定。结果当小柴胡汤提取剂浓度在10
The normal skin of the patient was taken from the operation and Xiaochaihu Decoction was added to the serum-free medium to culture proliferating cells. The activity of IL-1α and IL-6 in the supernatant was determined by enzyme-linked immunosorbent assay (ELISA). The toxicity of Xiaochaihu decoction was measured by MTT assay. When the concentration of Xiaochaihu Tang extractant is 10