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为了揭示连作条件下植烟土壤细菌群落结构及其与土壤环境间的响应关系,本研究采用了Illumina平台Hiseq2500高通量测序技术,对不同施肥处理(常规施肥,蚯蚓粪肥,微生物菌肥以及蚯蚓粪和微生物菌肥混合)的漯河烟区连作植烟土壤细菌进行16S r RNA V4区测序,结合冗余分析(redundancy analysis,RDA)研究土壤细菌微生物的群落结构组成、多样性以及与土壤环境间的相关关系。结果表明,测序质控后共获得25 203个操作分类单元(operational taxonomic units,OTUs),计1 600 239条读数。分层聚类图显示,不同施肥处理的连作植烟土壤细菌群落有较大差异,但这种差异不体现在结构多样性上。多样性指数分析表明,连作植烟土壤细菌群落易受环境变化的影响,体现出一定的时间差异性;烤烟成熟期土壤丰度指数明显升高,微生物菌肥和蚯蚓粪肥处理下土壤细菌群落丰度变化较大。主成分分析表明,不同土壤环境因子间有很强的相关关系,可以将原有的11个土壤环境因子按照强正相关关系划分为4类;RDA结果表明,土壤p H既影响土壤细菌群落的多样性,又影响土壤细菌群落的丰度;而有机质主要对土壤细菌群落丰度有积极影响。研究结果为在微生物水平上研究连作植烟障碍的形成机理提供了依据。
In order to reveal the bacterial community structure and its response to soil environment under continuous cropping conditions, Illumina platform Hiseq2500 high-throughput sequencing was used in this study. The effects of different fertilization treatments (conventional fertilization, earthworm manure, microbial fertilizer and earthworm Feces and microbial fertilizer) in the Luohe tobacco-growing areas of continuous cropping tobacco soil bacterial 16S r RNA V4 district sequencing, combined with redundancy analysis (redundancy analysis, RDA) study of microbial community composition of soil bacteria, diversity and soil environment The relationship between. The results showed that a total of 25 203 operational taxonomic units (OTUs) were obtained after sequencing and quality control, accounting for 1 600 239 readings. Hierarchical cluster maps showed that there were great differences in bacterial communities in continuous cropping tobacco with different fertilization treatments, but this difference was not reflected in the structural diversity. The analysis of diversity index showed that the bacterial community in continuous cropping tobacco was susceptible to environmental changes and showed some time differences. The soil abundance index at maturity of flue-cured tobacco significantly increased. The microbial community abundance under microbial and earthworm manure treatments Degree changes larger. Principal component analysis showed that there was a strong correlation between different soil environmental factors, and the original 11 soil environmental factors could be classified into 4 categories according to a strong positive correlation. The RDA results showed that soil p H affected both the bacterial community Diversity, but also affect the abundance of soil bacterial community; and organic matter mainly has a positive impact on soil bacterial community abundance. The results provided the basis for studying the formation mechanism of continuous cropping tobacco disturbance at the level of microorganisms.