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和其他诱变方法相比较,低能氮离子束注入作为一种新的诱变方法具有生理损伤小、突变谱广和突变频率高等特点。据此利用该方法处理“遗4212”,建立起具有60个株系的突变群体。通过调查生育期、农艺性状、醇溶蛋白和微卫星的变异,对后代M4群体进行系统的研究。结果表明:群体的生育期和农艺性状变异明显,有7个ω-醇溶蛋白的迁移率变异并伴随着蛋白的缺失和增加;在25个SSR位点出现扩增产物的缺失、延长和缩短。结合实验结果和其他相关报道,讨论了实验所获得突变体的应用及离子束注入引发突变的机理。
Compared with other mutagenesis methods, low-energy nitrogen ion implantation has the characteristics of small physiological damage, broad mutation spectrum and high mutation frequency as a new mutagenesis method. According to this method, “QI 4212” was processed and a mutant population with 60 lines was established. The offspring M4 population was studied systematically by investigating the variation of growth stage, agronomic traits, gliadin and microsatellite. The results showed that there were obvious variation in the growth and agronomic traits of the population with seven ω-gliadin mobility variations accompanied by the deletion and increase of the protein. The deletion, prolongation and shortening of the amplification products appeared in 25 SSR loci . Combined with the experimental results and other relevant reports, we discussed the application of mutants and the mechanism of ion beam-induced mutation.