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目的探讨抗角蛋白自身抗体(AK auto Ab)对角质形成细胞凋亡的影响。方法以不同浓度AK auto Ab作用体外培养人角质形成细胞后,用光镜及透射电镜观察细胞形态变化、用流式细胞仪分析细胞周期变化以及提取角质形成细胞DNA作电泳特征分析。结果 AK auto Ab作用后培养的角质形成细胞光镜、电镜下形态发生凋亡特有的改变,出现核固缩、染色质凝聚,并形成凋亡小体；细胞周期分析图中出现凋亡峰；DNA电泳呈有一定间隔的梯状条带。结论 AK auto Ab对角质形成细胞的凋亡具有诱导作用。
Objective To investigate the effect of anti-keratin autoantibodies (AK auto Ab) on keratinocyte apoptosis. Methods Human keratinocytes were cultured in vitro with different concentrations of Ak auto Ab. Morphological changes of cells were observed under light microscope and transmission electron microscopy. Cell cycle analysis was performed by flow cytometry and DNA was extracted from keratinocytes for electrophoretic analysis. Results Keratinocytes cultured under the action of AK auto Ab were observed under light microscopy. The morphological changes of nuclei were observed under electron microscope. Nuclear condensation and chromatin condensation were observed, and apoptotic bodies were formed. Apoptotic peak appeared in the cell cycle analysis. DNA electrophoresis showed a certain interval of ladder-shaped band. Conclusion AK auto Ab induces the apoptosis of keratinocytes.