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目的 探讨正糖 高胰岛素钳夹及游离脂肪酸 (FFA)对大鼠血浆ghrelin浓度的影响。方法 建立清醒状态下大鼠正常血糖 高胰岛素钳夹技术 ,在钳夹前后分别测定大鼠血浆ghrelin浓度 ,脂质灌注组大鼠 12只 ,生理盐水组 12只为对照 ,并用 3 3 H葡萄糖作为示踪剂测定了胰岛素介导的外周和肝糖的代谢。结果 在胰岛素钳夹中 ,脂质灌注组大鼠血浆FFA明显增加 (从 74 2 μmol/L±5 1μmol/L到 2 346 μmol/L± 2 38μmol/L ,P <0 0 1)。脂质灌注组葡萄糖输注率 (GIR)明显低于对照组( 2 0 0 2 4 0min ,平均 12 6mg·kg-1·min-1± 1 5mg·kg-1·min-1vs 34 0mg·kg-1·min-1± 1.6mg·kg-1·min-1,P <0 0 1)。到钳夹结束时 ,下降至相应对照值的 35 % ( 2 4 0min ,12 0mg·kg-1·min-1± 1 9mg·kg-1·min-1vs 34 7mg·kg-1·min-1± 1 7mg·kg-1·min-1,P <0 0 1)。对照组肝糖产率 (HGP)明显被抑制 ( 88% ) ,从 19 0mg·kg-1·min-1± 4 5mg·kg-1·min-1降至 2 3mg·kg-1·min-1± 0 9mg·kg-1·min-1(P <0 0 1)。脂质输注组胰岛素对HGP的抑制作用明显障碍 ( 2 0 0 2 4 0min ,从 18 7mg·kg-1·min-1± 3 0mg·kg-1·min-1到 2 3 2mg·kg-1·min-1± 3 1mg·kg-1·min-1,P <0 0 5 )。钳夹术结束时 ,对照
Objective To investigate the effect of normal glucose hyperinsulinemic clamp and free fatty acid (FFA) on plasma ghrelin concentration in rats. Methods The normal high blood glucose insulin clamp technique was established in conscious rats. Plasma ghrelin concentration was measured before and after the clamp in rats. Twelve rats in the lipid-perfusion group and 12 rats in the saline group were used as controls. The levels of ghrelin Tracer measures insulin-mediated peripheral and glycogen metabolism. Results In the insulin clamp, plasma FFA increased significantly (from 74 2 μmol / L ± 5 1 μmol / L to 2 346 μmol / L ± 2 38 μmol / L, P <0.01) in the lipid perfused rats. The glucose infusion rate (GIR) in the lipid-perfusion group was significantly lower than that in the control group (mean of 120 mg · kg-1 · min-1 ± 15 mg · kg-1 · min-1 vs 34 mg · kg- -1 · min -1 ± 1.6 mg · kg -1 · min -1, P <0.01). At the end of the clamp, it dropped to 35% of the corresponding control value (240 min, 120 mg kg -1 · min -1 ± 1 9 mg · kg -1 · min -1 vs 34 7 mg · kg -1 · min -1 ± 17mg · kg-1 · min-1, P <0.01). The glycogen yield (HGP) in the control group was significantly reduced (88%) from 19 0 mg · kg -1 · min -1 ± 45 mg · kg -1 · min -1 to 23 mg · kg -1 · min -1, 1 ± 0 9 mg · kg -1 · min -1 (P 0 01). The inhibitory effect of insulin on lipid peroxidation in rats in the lipid infusion group was obviously obstructed (from 20 7 2 0 min, from 18 7 mg · kg -1 · min-1 ± 30 mg · kg-1 · min-1 to 23 2 mg · kg- 1 · min-1 ± 3 1 mg · kg-1 · min-1, P <0 05). At the end of the clamp, the control