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目的观察不同浓度棕榈酸培养INS-1细胞时过氧化物酶体增殖物激活受体γ辅助激活因子-1α(PGC-1α)的表达变化及其与细胞增殖、凋亡和胰岛素分泌能力的关系。方法 INS-1细胞在50、100、400μmol/L棕榈酸培养4、8、12、24、48h检测PGC-1αmRNA和蛋白表达、细胞增殖活力、Bcl-2蛋白、基础及葡萄糖刺激的胰岛素分泌。结果 50μmol/L棕榈酸培养时,INS-1细胞PGC-1αmRNA48h有增多趋势,但差异无统计学意义;8、12h细胞增殖活力降低,24、48h与对照组比较差异无统计学意义;Bcl-2蛋白表达无变化;48h基础及葡萄糖刺激胰岛素分泌量增加。100μmol/L棕榈酸培养时,INS-1细胞PGC-1αmRNA 24h表达增加,48h蛋白表达增加;8、12、24h细胞增殖活力降低,48h与对照组比较差异无统计学意义;Bcl-2蛋白表达无变化;基础及葡萄糖刺激胰岛素分泌量增加。400μmol/L棕榈酸培养时,INS-1细胞PGC-1αmRNA 12h表达增加,24h后PGC-1α蛋白表达进行性增加;8h起细胞增殖活力降低;24h起Bcl-2蛋白表达减少;48h基础胰岛素分泌及葡萄糖刺激的胰岛素分泌降低。结论轻、中度高脂培养条件下INS-1细胞PGC-1α表达轻度增加,与细胞增殖活力改变和胰岛素分泌能力增加相关,重度高脂培养条件下INS-1细胞PGC-1α表达增加,与细胞增殖活力减低、抗凋亡减少和胰岛素分泌能力受损相关,提示PGC-1α可能参与棕榈酸对INS-1细胞增殖、凋亡及功能改变的影响。
Objective To observe the expression of peroxisome proliferator-activated receptor γ-activated coactivator-1α (PGC-1α) in INS-1 cells incubated with different concentrations of palmitic acid and its relationship with cell proliferation, apoptosis and insulin secretion . Methods INS-1 cells were cultured in 50,100,400μmol / L palmitic acid for 4, 8, 12, 24, 48 hours to detect PGC-1αmRNA and protein expression, cell proliferation activity, Bcl-2 protein, basal and glucose stimulated insulin secretion. Results When PGCs were treated with 50μmol / L palmitic acid for 48h, PGC-1αmRNA 48h showed an increasing tendency, but the difference was not statistically significant. The proliferation activities of cells at 8 and 12h were lower than that of the control group, while there was no significant difference between the two groups 2 protein expression did not change; 48h basal and glucose-stimulated insulin secretion increased. At 100μmol / L palmitic acid, the expression of PGC-1α mRNA in INS-1 cells increased at 24h and increased at 48h, while at 8, 12 and 24 hours, there was no significant difference between the two groups No change; basal and glucose-stimulated insulin secretion increased. At 400μmol / L palmitic acid, the expression of PGC-1αmRNA in INS-1 cells increased at 12h, and the expression of PGC-1αprotein increased progressively at 24h, and decreased at 8h; the expression of Bcl-2 decreased at 24h; the basal insulin secretion at 48h And glucose-stimulated insulin secretion decreased. Conclusions The expression of PGC-1α in INS-1 cells is slightly increased under mild and moderate hyperlipidemic conditions, which is related to the change of cell proliferation and insulin secretion. The expression of PGC-1α in INS-1 cells is increased under severe hyperlipidemic conditions, Which is related to the decrease of cell proliferation activity, anti-apoptosis and impaired insulin secretion, suggesting that PGC-1α may be involved in the effect of palmitate on the proliferation, apoptosis and function of INS-1 cells.