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目的了解山西省地区乙型肝炎病毒(HBV)基因型的分布特征,探讨HBV各基因型与血清HBV-DNA水平的关系以及各基因型与疾病的进展和转归的临床意义。方法法随机收集山西省HBV阳性病例128例,包括慢性乙型肝炎78例,肝硬化34例,急性肝炎8例,重型肝炎患者8例。取其外周静脉血,采用型特异性引物巢式PCR方法对血清中的HBV进行基因分型,并结合临床资料进行统计学分析结果山西省地区乙型肝炎患者HBV基因分型存在B型、B+C混合型和C型,以C型和B+C型为主;基因型C型的HBV-DNA载量的均值水平为2×108,显著高于其他型(p<0.05);C型HBeAg阳性率较B型和B+C型高(p<0.05);混合型及C基因型与较严重的肝脏疾病有关,更易发生重症肝炎、肝硬化。结论山西省地区基因型以C型和B+C型为主;基因型C的HBV-DNA载量水平均值显著高于其他型;混合型及C型与较严重的肝脏疾病有关。
Objective To understand the distribution of hepatitis B virus (HBV) genotypes in Shanxi Province and to explore the relationship between HBV genotypes and serum HBV-DNA levels and the clinical significance of the progress and outcome of each genotype and disease. Methods Randomly collected 128 cases of HBV-positive cases in Shanxi Province, including 78 cases of chronic hepatitis B, 34 cases of cirrhosis, 8 cases of acute hepatitis and 8 cases of severe hepatitis. Take the peripheral venous blood, using type-specific primers nested PCR method for HBV genotyping in serum and statistical analysis of clinical data combined results of HBV genotypes in patients with hepatitis B in Shanxi Province there B, B + C mixed type and C type, mainly C type and B + C type. The average level of HBV-DNA load of genotype C type was 2 × 108, which was significantly higher than other types (p <0.05) The positive rate of HBeAg was higher than that of B and B + C (p <0.05). The mixed and C genotypes were associated with more severe liver diseases and were more likely to develop severe hepatitis and liver cirrhosis. Conclusion The genotypes of Shanxi Province are mainly C and B + C genotypes; HBV genotype C has significantly higher mean HBV DNA load levels than other genotypes; and mixed and C genotypes are associated with more severe liver diseases.