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本研究利用基因芯片技术检测白血病多药耐药细胞株HL-60/VCR及其亲本细胞株差异基因表达谱,探讨急性白血病多药耐药机制。本试验提取白血病细胞株HL-60及其多药耐药细胞株HL-60/VCR的mRNA,在反转录及体外转录过程中分别用生物素进行标记,与Affymetrix公司人类全基因组芯片U133A杂交,用Affymetrix专用芯片扫描仪G2500AGeneArrayScanner及MicroarraySuite、MicroDBTMGeneChipLIMS、GeneChipDMT分析软件系统处理杂交信号获取结果。结果表明:白血病细胞株HL-60及其耐药株HL-60/VCR差异显示基因5507条,其中耐药细胞株中上调表达基因3100条,下调表达基因2407条;差异极显著性基因1040条,表达上调435条,下调605条,涉及与细胞生长活动及信号转导相关的基因。结论:多基因参与白血病细胞株的多药耐药机制,基因芯片技术是并行分析多个基因、探讨白血病多药耐药机制的有效方法。
In this study, differential gene expression profiles of HL-60 / VCR cells and their parental cell lines were detected by gene chip technology to investigate the multidrug resistance mechanism of acute leukemia. In this study, leukemia cell line HL-60 and multidrug-resistant cell line HL-60 / VCR mRNA were extracted, respectively labeled with biotin during reverse transcription and in vitro transcription, and then hybridized with Affymetrix human whole genome chip U133A , With Affymetrix chip scanner G2500AGeneArrayScanner and MicroarraySuite, MicroDBTMGeneChipLIMS, GeneChipDMT analysis software system to deal with hybridization signal acquisition results. The results showed that there were 5507 genes in leukemia cell line HL-60 and their resistant strains HL-60 / VCR, of which 3100 genes were up-regulated and 2,407 genes were down-regulated in drug-resistant cell lines; 1040 genes with extremely different significance , Up-regulated 435, down-regulated 605, related to cell growth activity and signal transduction related genes. Conclusion: Multi-gene is involved in the multi-drug resistance mechanism of leukemia cell lines. Gene chip technology is an effective method to analyze multiple genes in parallel and explore the multi-drug resistance mechanism of leukemia.