建立了北刘寄奴药材中木犀草素和毛蕊花糖苷的反相高效液相含量测定方法。采用Agilent Zorbax SB-Aq C18 column (250 mm×4.6 mm, 5 μm) 色谱柱; 0.05%磷酸 (A) 和甲醇 (B) 梯度洗脱; 流速1.0 mL/min; 检测波长310 nm和350 nm。木犀草素和毛蕊花糖苷分别在0.0341-0.8172 mg/mL (r2 = 0.9999)和0.0708-2.832 mg/mL (r2 = 0.9999)范围内与峰面积呈良好的线性关系。平均加样回收率分别为102.7%和98.3%。15批北刘寄奴药材中木犀草素和毛蕊花糖苷的含量差异明显。本文首次报道了北刘寄奴药材中毛蕊花糖苷的含量测定方法。 The method for the determination of luteolin and verbascoside in Beiliuhuangniu was established. The column was eluted with a gradient of 0.05% phosphoric acid (A) and methanol (B) using an Agilent Zorbax SB-Aq C18 column (250 mm × 4.6 mm, 5 μm) with a flow rate of 1.0 mL / min and detection wavelengths of 310 nm and 350 nm. Luteolin and Verbascoside showed a good linear relationship with the peak area in the range of 0.0341-0.8172 mg / mL (r2 = 0.9999) and 0.0708-2.832 mg / mL (r2 = 0.9999), respectively. The average recovery rates were 102.7% and 98.3% respectively. The contents of luteolin and verbascoside in 15 batches of North Liu slave were significantly different. This article for the first time reported Bei Liu Nuhu herbs Vermiculidin determination of content.