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本文采用双抗体夹心ＥＬＩＳＡ法分别测定了２１例反复呼吸道感染儿，３０例正常儿童，１０例新生儿脐血的血清可溶性白细胞介素２受体（ｓＩＬ＝２Ｒ）水平。结果患儿组ｓＩＬ－２Ｒ为７１６．６０±３０．１０Ｕ／ｍｌ；正常儿童组为３８４．４７±８８．０３Ｕ／ｍｌ（ｐ＜０．０１）；新生儿脐血为４４６．２０±５５．６８Ｕ／ｍｌ，与正常儿童比较Ｐ＞０．０５。同时采用间接免疫荧光技术测定了患儿Ｔ细胞亚群水平，结果ＣＤ８细胞数升高，ＣＤ３细胞和ＣＤ４细胞数、ＣＤ４／ＣＤ８比值下降，与正常儿童比较有显著性差别。提示反复呼吸道感染儿有细胞免疫功能降低及免疫调节紊乱。
The serum soluble interleukin 2 receptor (sIL = 2R) levels in 21 cases of recurrent respiratory tract infection, 30 cases of normal children and 10 cases of newborns were determined by double antibody sandwich ELISA. Results The sIL-2R in children group was 716.60 ± 30.10U / ml, that in normal children group was 384.47 ± 88.03U / ml (p <0.01), and the neonatal cord blood was 446.20 ± 55. 68U / ml, compared with normal children P> 0.05. At the same time, indirect immunofluorescence assay was used to measure the level of T lymphocyte subsets in children. As a result, the number of CD8 cells increased, the number of CD3 cells, CD4 cells and the ratio of CD4 / CD8 decreased. Compared with normal children, there was a significant difference. Prompt recurrent respiratory tract infection in children with reduced cellular immune function and immune disorders.