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目的 探讨乙型肝炎病毒 (HBV)感染与IgA肾病的关系。方法 采用免疫组化技术检测肾组织HBV抗原 (HBAg) ,应用原位分子杂交技术和Southern印迹杂交技术检测肾组织HBVDNA。结果 1 0 0例IgA肾病患者中 ,血清HBsAg阳性 1 8例 (1 8% ) ;肾组织HBAg阳性 31例 (31 % ) ,HBAg在肾小球中阳性率为 65 % (2 0 /31 ) ,其中HBsAg为 32 % (1 0 /31 ) ,HBcAg为 32 % (1 0 /31 ) ;除肾小球外 ,HBcAg在肾小管上皮细胞和肾间质亦有阳性沉积 ,分别为 1 4例 (45 % )和 2例 (6 % ) ;Southern印迹杂交证实 6例患者中 5例肾组织有整合型HBVDNA ;原位分子杂交证实肾小管和肾小球中HBVDNA阳性率分别为 1 0 0 % (8/8)和 75 % (6/8) ;定位于肾小管上皮细胞、肾小球细胞和肾间质浸润的淋巴细胞中。结论 HBV感染与IgA肾病密切相关 ,HBV可能参与了IgA肾病的发病过程
Objective To investigate the relationship between hepatitis B virus (HBV) infection and IgA nephropathy. Methods Kidney HBV antigen (HBAg) was detected by immunohistochemistry. HBVDNA was detected by in situ hybridization and Southern blotting. Results Among 100 IgA nephropathy cases, 18 (18%) were positive for HBsAg, 31 (31%) were positive for HBAg in renal tissue, and 65% (2/31) were positive for glomerular HBAg , HBsAg was 32% (10/31) and HBcAg was 32% (10/31). In addition to glomerular, HBcAg also positive deposition in renal tubular epithelial cells and renal interstitium were 14 cases (45%) and 2 cases (6%). Southern blot hybridization confirmed that 5 of 6 patients had integrated HBVDNA. The positive rate of HBVDNA in renal tubules and glomerulus by in situ hybridization was 100% (8/8) and 75% (6/8) respectively. It was located in the lymphocytes infiltrated by renal tubular epithelial cells, glomerular cells and renal interstitium. Conclusion HBV infection is closely related to IgA nephropathy. HBV may be involved in the pathogenesis of IgA nephropathy