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子宫平滑肌瘤是最常见的子宫良性肿瘤,在30岁以上的妇女中发病率为20～30%恶变率则低于1%,以前的文献显示,其7,12,14号染色体最常发生重排。我们对2例子宫平滑肌瘤进行了核型分析。肿瘤标本经充分剪碎后置胶原酶(200μ1/ml)中过夜,然后将悬液接种于含17%胎牛血清的RPMI64O培养基中,置5%CO_2的环境中培养5～9天,收获前用秋水仙素(0.01μl/ml)处理过夜,常规制片,GTG显带。
Uterine leiomyomas are the most common benign tumors of the uterus. The incidence rate of 20 to 30% in women over the age of 30 is less than 1%. The previous literature showed that chromosomes 7, 12 and 14 were the most common. rearrange. We performed karyotype analysis of 2 cases of uterine leiomyoma. The tumor specimens were fully smashed and placed in collagenase (200μ1/ml) overnight, and then the suspension was inoculated into RPMI64O medium containing 17% fetal bovine serum and cultured in a 5% CO 2 environment for 5 to 9 days. Before treatment with colchicine (0.01μl/ml), conventional production, GTG banding.