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目的:探讨应用自体骨髓间充质干细胞(MSCs)移植治疗神经系统疾病的临床疗效。方法:收集患者24例,包括持续植物状态(PVS)12例、运动神经元病11例、桥脑小脑萎缩1例。入院后经骨髓穿刺采集自体骨髓,当天分离回输1×108个单个核细胞,剩余细胞进行MSCs培养扩增,待MSCs数量增至约1×108个细胞后收集细胞,经腰椎穿刺推注。治疗前后对患者神经功能变化分别进行评价。结果:12例PVS患者中,PVS评分提高、肌力改善5例;11例运动神经元病患者和1例橄榄桥脑小脑萎缩患者中,肌力、构音障碍改善5例。结论:MSCs移植治疗可以部分缓解神经系统疾病的症状,近期疗效明显,无不良反应,远期疗效还需进一步随访和观察。
Objective: To investigate the clinical efficacy of autologous bone marrow mesenchymal stem cells (MSCs) transplantation in the treatment of neurological diseases. Methods: Twenty-four patients were collected, including 12 cases of persistent vegetative state (PVS), 11 cases of motor neuron disease and 1 case of pontine cerebellar atrophy. Admitted to the hospital after bone marrow puncture acquisition of autologous bone marrow, the same day separated and transfused 1 × 108 mononuclear cells, the remaining cells were cultured MSCs expansion until MSCs increased to about 1 × 108 cells collected after lumbar puncture. Before and after treatment of patients with neurological changes were evaluated. Results: In 12 PVS patients, the PVS score was improved and the muscle strength was improved in 5 cases. Among the 11 patients with motor neuron disease and 1 patient with olivopontocerebellar atrophy, the muscle strength and dysarthria were improved in 5 cases. Conclusion: The transplantation of MSCs can partially relieve the symptoms of neurological diseases. The curative effect is obvious in the near future without any adverse reaction. The long-term curative effect needs further follow-up and observation.