论文部分内容阅读
对甲基硝基亚硝基胍（ＭＮＮＧ）在诱发体外培养的人胃粘膜上皮细胞恶性转化中的作用进行了观察，结果发现，ＭＮＮＧ处理组各时相胃粘膜上皮细胞的增殖速率，非程序ＤＮＡ合成水平，脂质过氧化物和ｒａｓｐ２１蛋白含量均显著高于对照组（Ｐ＜０．０５～０．０１）。结果提示，ＭＮＮＧ可诱发胃粘膜上皮细胞恶性转化，其作用机理可能是通过损伤细胞ＤＮＡ，诱发脂质过氧化或导致ｒａｓｐ２１异常表达所致。
The role of MNNG in the induction of malignant transformation of human gastric epithelial cells cultured in vitro was observed. The results showed that the proliferation rate of gastric epithelial cells at various phases of the MNNG-treated group was not a procedure. The level of DNA synthesis, lipid peroxidation and rasp21 protein content were significantly higher than those in the control group (P<0.05-0.01). The results suggest that MNNG can induce the malignant transformation of gastric epithelial cells. The mechanism may be caused by the damage of cellular DNA, induce lipid peroxidation or lead to abnormal expression of rasp21.