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热点是体细胞染色体频繁地出现自发或某些因素诱发的断裂或裂隙的部位。在缺叶酸和胸苷的培养条件下,染色体热点和大部分脆点频率显著上升,其原因可能为DNA中尿苷错误地取代胸苷之故。为了证明这一假设,实验从16个健康的成人(6男、10女)和9个孕妇取得外周淋巴细胞,培养于缺叶酸和胸苷的培养基(MEM-FA)中。在培养结束前24或72小时,加入2mM、4mM和8mM尿苷。培养96时小制片。以不加尿苷的细胞为对照。 实验结果表明,不加尿苷的细胞,热点3p14频率为1.23/100(2,450个细胞),加入2、4和8mM尿苷后,热点3p14频率分别提高到9.20±2.22,9.13±2.34和11.33±3.43。各组和对照间有极显著的差异。热点16q23有相似的
Hot spots are frequent occurrence of spontaneous or some factor-induced fracture or crevice of somatic chromosomes. In the absence of folic acid and thymidine culture conditions, chromosome hot spots and most of the brittle point frequency increased significantly, the reason may be wrong to replace uridine DNA in the thymidine. To test this hypothesis, peripheral lymphocytes were obtained from 16 healthy adults (6 males and 10 females) and 9 pregnant women and cultured in medium containing folate and thymidine (MEM-FA). At 24 or 72 hours before the end of the culture, 2 mM, 4 mM and 8 mM uridine were added. 96 hours of small film. Ugly-free cells as controls. The results showed that the frequency of hot 3p14 was 1.23 / 100 (2,450 cells) without uridine, and the hot 3p14 frequency increased to 9.20 ± 2.22, 9.13 ± 2.34 and 11.33 ± 3.43. There were significant differences between groups and controls. Hot 16q23 have similar