论文部分内容阅读
背景:间充质干细胞是具有自我更新能力且能够多向分化的干细胞。脐带属于胚胎外组织,是胎儿分娩后的废弃物,来源广泛,无伦理学限制,因此有望作为间充质干细胞来源的第一选择。目的:检测人脐带间充质干细胞冻存复苏前后细胞表面分子CD29、CD44、CD49e、CD73、CD90、CD34、CD45、CD271标志变化情况。方法:通过从人的脐带中分离培养间充质干细胞,分别观察原代细胞、P4、P8代细胞和冻存复苏后P2、P4、P8代细胞形态;通过流式细胞仪检测原代细胞、P4、P8代细胞和冻存复苏后P2、P4、P8代细胞表面分子标志CD29、CD44、CD49e、CD73、CD90、CD34、CD45、CD271。结果与结论:人脐带间充质干细胞冻存前与原代复苏后不同代数下细胞均表现出相同的表型,CD29、CD44、CD49e、CD73、CD90阳性,CD34、CD45、CD271阴性,提示人脐带间充质干细胞原代低温冻存复苏后细胞表面标志性分子无变化。
Background: Mesenchymal stem cells are stem cells that are self-renewing and capable of multiple differentiation. The umbilical cord belongs to the extraembryonic tissue. It is a waste after the birth of the fetus. Due to its wide range of sources and no ethical limitations, the umbilical cord is expected to be the first choice of mesenchymal stem cell source. Objective: To detect the changes of cell surface markers CD29, CD44, CD49e, CD73, CD90, CD34, CD45 and CD271 before and after human umbilical cord mesenchymal stem cells cryopreservation and resuscitation. Methods: Mesenchymal stem cells (MSCs) were isolated and cultured from human umbilical cord. The morphological changes of primary cells, P4 and P8 generation and P2, P4 and P8 generation after cryopreservation and resuscitation were observed respectively. Primary cells were detected by flow cytometry, The cell surface markers CD29, CD44, CD49e, CD73, CD90, CD34, CD45 and CD271 on P4, P8 and P2, P4 and P8 after cryopreservation and resuscitation. RESULTS AND CONCLUSION: Human umbilical cord mesenchymal stem cells showed the same phenotype before and after primary resuscitation, with positive expression of CD29, CD44, CD49e, CD73 and CD90, but negative for CD34, CD45 and CD271 Umbilical cord mesenchymal stem cells after primary cryogenic preservation of cell surface markers did not change.