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人血谷胱甘肽过氧化物酶(GPx)与硒含量之比显著低于各种动物,但人与动物的红细胞含硒量却类似。提示人与动物的血浆及红细胞蛋白中的硒分布是不同的。就人类而言,红细胞中的硒大部份与血红蛋白结合,而大多数动物的红细胞硒却与GPx 结合。此种差异的原因可能是由于人与动物所消耗的硒的化学形态不同所致,而未必是由于种属的不同。作者的研究表明,用亚硒酸盐及硒蛋氨酸分别喂大鼠,其血的凝胶过滤显示差异。当用亚硒酸盐时,大部份硒从红细胞 GPx 中洗脱出来,反之,用硒蛋氨酸时,大部份硒从红细胞血红蛋白中洗脱出来。当以高硒小麦或富硒酵母作大鼠饲料硒来源时,大部份硒存在于红细胞的血红蛋白中,提示,硒的化学形态对动物红细胞蛋白之间的硒分布具有明显的影响。当血浆凝胶过滤时,动物仅有一个硒峰,而人却有两个。但人的两个硒峰都不带有GPx。当给罗猴补充亚硒酸盐时,仅得到一个凝胶过滤峰,当饮水中含硒蛋氨酸时,就可得到二个含硒峰。与人的血浆一样,GPx 也不从这二个硒峰中洗脱出来。已经证实,第一个硒峰是硒蛋白 P,而第二个硒峰是白蛋白。因此,作者推测,人与动物红细胞及血浆蛋白中硒分布差异的原因是食品中硒的主要形式是硒蛋氨酸。本文还对克山病除硒以外可能涉及的其它一些因素进行了讨论。
Human blood glutathione peroxidase (GPx) and selenium content ratio was significantly lower than a variety of animals, but human and animal erythrocytes selenium content is similar. Suggesting that human and animal plasma and red blood cell protein distribution of selenium is different. In humans, most of the selenium in red blood cells binds to hemoglobin, whereas selenium in most animals binds to GPx. This difference may be due to human and animal consumption of selenium due to different chemical forms, not necessarily due to the different species. The authors study showed that the selenium and selenomethionine fed rats, the blood gel filtration showed differences. When selenite is used, most of the selenium elutes from the erythrocyte GPx, whereas when selenomethionine is used, most of the selenium elutes from erythrocyte hemoglobin. When selenium or selenium-enriched yeast was used as the source of selenium in rats, most of the selenium was present in the hemoglobin of erythrocytes, suggesting that the chemical form of selenium has a significant effect on selenium distribution among animal red blood cell proteins. When plasma gel filtration, animals have only one selenium peak, while humans have two. But neither of the two selenium peaks carry GPx. When rhesus monkey is supplemented with selenite, only one gel filtration peak is obtained, and two selenium peaks are obtained when selenomethionine is contained in drinking water. Like human plasma, GPx does not elute from these two selenium peaks. It has been confirmed that the first selenium peak is selenoprotein P and the second selenium peak is albumin. Therefore, the authors speculate that the reason for the difference in the distribution of selenium in human and animal erythrocytes and plasma proteins is that the main form of selenium in foods is selenomethionine. This article also discusses other factors that may be involved in addition to selenium in Keshan disease.