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建立了反相高效液相色谱法测定婴幼儿配方食品中叶黄素测定方法。选用色谱柱YMC Carotenoid4.6mm×250mm,5μm,以甲醇、甲基叔丁基醚为流动相进行梯度洗脱,流速为1mL/min,于445nm波长下,测定婴幼儿配方食品中叶黄素的含量,叶黄素含量在0.0164～0.492μg/mL范围内呈线性关系,R=0.9998,平均回收率为99.2%,RSD为1.1%。该方法可以简便准确的测定婴幼儿配方食品中叶黄素的含量。
A RP-HPLC method for the determination of lutein in infant formula was established. The chromatographic column YMC Carotenoid 4.6 mm × 250 mm, 5 μm, gradient elution with methanol and methyl tert-butyl ether was used as the mobile phase, the flow rate was 1 mL / min, and the content of lutein in infant formula was determined at 445 nm , Lutein content in the range of 0.0164 ~ 0.492μg / mL showed a linear relationship, R = 0.9998, the average recovery was 99.2%, RSD was 1.1%. The method can be simple and accurate determination of infant formula lutein content.