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[目的]了解HIV阳性感染者宫颈脱落细胞P16INK4a蛋白表达与HPV感染的相关性。[方法]采用通用引物PCR和反向点杂交技术建立的检测方法,对182例HIV阳性感染者和300例HIV阴性感染者检测23种HPV亚型,包括18种高危型和5种低危型。同时采用免疫组织化学技术(S-P染色法)检测宫颈脱落细胞中P16INK4a蛋白表达情况。[结果]HIV阳性女性HPV感染率(36.8%)及P16INK4a蛋白阳性表达率(18.7%)较HIV阴性者(19.3%、4.3%)高(P均为0.000)。HIV阳性患者中,HPV感染阳性者宫颈脱落细胞P16INK4a蛋白表达率为43.3%,HPV阴性者4.3%(P=0.000);HPV多型感染者宫颈脱落细胞P16INK4a蛋白表达率(60.9%)较HPV单型感染者高(34.1%)(P=0.042);高危型HPV感染者宫颈脱落细胞P16INK4a蛋白表达率(80.6%)较低危型HPV感染者高(0)(P=0.000)。HIV阴性患者中,HPV感染阳性者P16INK4a蛋白阳性表达率(5.2%)较HPV阴性者(1.3%)高(P=0.721);所检HPV阴性患者均为单一HPV感染;高危型HPV感染患者P16INK4a蛋白阳性表达率(40.0%)较低危型(1.9%)明显增高(P=0.018)。[结论]HIV阳性女性中HPV感染阳性、多型HPV感染及高危型HPV感染宫颈细胞P16INK4a蛋白的表达率更高。
[Objective] To investigate the correlation between the expression of P16INK4a protein and HPV infection in cervical exfoliated cells in HIV-positive patients. [Method] The detection methods of universal primer PCR and reverse dot blot were used to detect 23 HPV subtypes in 182 HIV-positive patients and 300 HIV-negative patients, including 18 high-risk and 5 low-risk . Immunohistochemistry (S-P staining) was used to detect the expression of P16INK4a protein in cervical exfoliated cells. [Results] The positive rates of HPV infection (36.8%) and P16INK4a (18.7%) in HIV positive women were higher than those in HIV negative people (19.3%, 4.3%) (P = 0.000). In HIV-positive patients, the positive rates of P16INK4a protein in cervical exfoliated cells were 43.3% and 4.3%, respectively (P = 0.000). The positive rates of P16INK4a protein expression in cervical exfoliated cells (60.9%) were significantly higher than those in HPV single positive (34.1%) (P = 0.042). The positive rate of P16INK4a protein in cervical exfoliated cells (80.6%) in high-risk HPV was higher than that in low-risk HPV (P = 0.000). Among HIV-negative patients, the positive rate of P16INK4a protein in HPV-positive patients was 5.2% higher than that in HPV-negative patients (1.3%) (P = 0.721) The positive expression rate of protein (40.0%) was significantly higher than that of lower type (1.9%) (P = 0.018). [Conclusion] The positive rate of P16INK4a protein in cervical cells was higher in HIV-positive women with HPV infection, multi-type HPV infection and high-risk HPV infection.