Arsenic (As) in paddy fields is deteriorating food security and human health through rice ingestion.Rice is the dominant food source of arsenic exposure to half of the world’s population.Therefore,an in situ effective method for As risk evaluation in paddy soil is strongly needed to avoid As exposure through rice ingestion.Herein,we developed a rapid analytical methodology for determination of As in plant tissues using field portable X-ray fluorescence spectrometry (FP-XRF).This method was applied to rice roots in order to evaluate the As contamination in paddy soils.The results showed that rice roots with iron plaques were superior to rhizosphere soils for generating FP-XRF signals,especially for field sites with As concentrations lower than the soil detection limit of FP-XRF (30.0 mg/kg).Moreover,the strong linear relationships of As concentrations between the rice roots and corresponding leaves and grains proved that the rice root,rather than the soil,is a better predictor of As concentrations in rice grains.The research provides an efficient As monitoring method for As contaminated paddy fields by using wetland plant roots with iron plaques and XRF-based analytical techniques.