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目的观察绿茶提取物茶多酚对硝酸羟胺染毒小鼠血常规、血浆生化值及病理形态学的影响。方法采用腹腔注射硝酸羟胺建立中毒模型。雄性ICR小鼠30只,随机分为正常组、硝酸羟胺染毒组、茶多酚治疗组,染毒前和染毒后1d、3d、5d和7d常规测量各组外周血细胞计数和血红蛋白值。染毒后第7天,动物再次称重后活杀,进行血浆生化指标和肝、肺病理学检查。结果经过统计学分析,茶多酚可明显减轻硝酸羟胺中毒导致的小鼠白细胞(WBC)、红细胞(RBC)、血小板(PLT)计数的改变,及谷草转氨酶/谷丙转氨酶(AST/ALT)、胆碱酯酶(CHE)、白蛋白(ALB)、乳酸脱氢酶(LDH)、尿酸(UA)、铁(Fe)的改变。肝、肺病理性改变以硝酸羟胺染毒组组织损伤最重,茶多酚治疗组改变较轻。结论茶多酚灌胃给药对硝酸羟胺中毒引起的氧化损伤具有一定的保护作用,主要病理形态学改变均较轻。
Objective To observe the effect of green tea extract tea polyphenols on blood routine, plasma biochemical value and pathological morphology of mice exposed to hydroxylamine nitrate. Methods The model of intoxication was established by intraperitoneal injection of hydroxylamine nitrate. Thirty male ICR mice were randomly divided into normal group, hydroxylamine nitrate exposure group, and tea polyphenols treatment group. Peripheral blood cell counts and hemoglobin values were routinely measured before and at 1 d, 3 d, 5 d, and 7 d after exposure. On the 7th day after exposure, the animals were weighed again and killed, and plasma biochemical indicators and liver and lung pathological examinations were performed. Results After statistical analysis, tea polyphenols can significantly reduce the changes of white blood cell (WBC), red blood cell (RBC), and platelet (PLT) counts caused by hydroxylamine nitrate poisoning, and aspartate aminotransferase/glutamate transaminase (AST/ALT), Changes in cholinesterase (CHE), albumin (ALB), lactate dehydrogenase (LDH), uric acid (UA), and iron (Fe). Liver and lung pathological changes were most severe with the hydroxylamine nitrate exposure group, and the tea polyphenols treatment group changed lighter. Conclusion The administration of tea polyphenols has a certain protective effect on oxidative damage caused by hydroxylamine nitrate poisoning. The main pathological changes are lighter.