目的:探讨血管内皮生长因子(VEGF)和可溶性血管内皮生长因子受体(sFlt-1)变化与子痫发病的关系,建立一种子痫前期的早期预测和诊断方法。方法:采用酶联免疫吸附法(ELISA)检测正常妊娠孕晚期和子痫前期、子痫期孕妇血清VEGF和sFlt-1水平,比较两者水平变化与病情进展的关系。结果:与正常妊娠组比较,子痫前期组VEGF显著低于正常水平,而sFlt-1显著高于正常水平。随子痫病情加重,VEGF水平呈逐步下降趋势,而sFlt-1水平呈逐步上升趋势。结论:孕妇血清VEGF的下降和sFlt-1的上升与子痫前期的发病及病情进展密切相关,可作为子痫的早期预测和判断指标。 Objective: To investigate the relationship between the changes of vascular endothelial growth factor (VEGF) and soluble vascular endothelial growth factor receptor (sFlt-1) and the pathogenesis of eclampsia and to establish an early prediction and diagnosis method of preeclampsia. Methods: Serum levels of VEGF and sFlt-1 in normal pregnant women and pregnant women with preeclampsia and preeclampsia were detected by enzyme-linked immunosorbent assay (ELISA), and their relationship with progression was compared. Results: Compared with normal pregnancy group, VEGF in preeclampsia group was significantly lower than normal level, while sFlt-1 was significantly higher than normal level. With the aggravation of eclampsia, the level of VEGF showed a gradual downward trend, while the level of sFlt-1 showed a gradual upward trend. Conclusion: The decrease of serum VEGF and the increase of sFlt-1 in pregnant women are closely related to the onset and progression of preeclampsia and may be used as an early predictor and indicator of eclampsia.