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[目的]探讨miR-143及其前体转录本在宫颈鳞癌中的表达及与临床病理特征的关系。[方法]采用Real-time PCR方法检测48例宫颈鳞癌及20例正常宫颈组织中miR-143的表达,分析miR-143与其前体转录本表达间的相关性,以及miR-143表达与宫颈鳞癌临床病理特征的关系。[结果]用Real-time PCR能有效检测miR-143及其前体转录本;miR-143及其前体转录本在宫颈鳞癌组织中的表达均显著低于正常宫颈组织(P<0.05),Spearman相关分析显示,miR-143前体转录本表达和miR-143表达呈明显正相关(r=0.723,P=0.000)。不同组织分化程度的宫颈鳞癌miR-143表达存在显著差异(P=0.005),小细胞型宫颈癌的miR-143表达明显低于其他组织分化类型的标本。miR-143的表达在不同年龄、肿块大小、大体类型及FIGO分期间未见显著差异(P>0.05)。[结论]前体转录本减少是miR-143表达下调的重要原因。miR-143在宫颈鳞癌组织中的异常表达,可能在宫颈鳞癌的发生和发展过程中发挥重要作用,有望成为宫颈鳞癌新的预后指标及治疗靶点。
[Objective] To investigate the expression of miR-143 and its precursor transcripts in cervical squamous cell carcinoma and its relationship with clinicopathological features. [Method] Real-time PCR was used to detect the expression of miR-143 in 48 cases of cervical squamous cell carcinoma and 20 cases of normal cervical tissue. The correlation between the expression of miR-143 and its precursor transcript and the relationship between miR-143 expression and cervical Relationship between clinicopathological features and squamous cell carcinoma [Result] Real-time PCR could effectively detect miR-143 and its precursor transcripts. The expression of miR-143 and its precursor transcripts in cervical squamous cell carcinoma tissues was significantly lower than that in normal cervical tissues (P <0.05) Spearman correlation analysis showed that miR-143 precursor transcripts were positively correlated with miR-143 expression (r = 0.723, P = 0.000). The expression of miR-143 in cervical squamous cell carcinoma with different histological grade was significantly different (P = 0.005). The expression of miR-143 in cervical squamous cell carcinoma was significantly lower than that in other tissue types. There was no significant difference in the expression of miR-143 between different age, tumor size, general type and FIGO stage (P> 0.05). [Conclusion] The decrease of precursor transcripts is an important reason for the down-regulation of miR-143 expression. The abnormal expression of miR-143 in cervical squamous cell carcinoma may play an important role in the occurrence and development of cervical squamous cell carcinoma, which is expected to become a new prognostic indicator and therapeutic target for cervical squamous cell carcinoma.