论文部分内容阅读
目的 :探讨丹参对创伤性急性肺损伤 (AL I)及多器官功能障碍综合征 (MODS)的治疗效应及可能机制。方法 :采用家兔创伤性 AL I模型 ,实验动物随机分为正常对照组 (8只 )、创伤性 AL I组 (8只 )和丹参治疗组(8只 ) ,进行各组动物动脉血气 ,血浆和支气管肺泡灌洗液 (BAL F)中肿瘤坏死因子 α(TNFα)含量及 BAL F中白细胞数量、补体 C3a、C5 a、白蛋白含量的测定。用免疫组织化学结合原位定量分析方法检测肺、肝、心、肾组织 TNFα蛋白表达、分布及相对含量 ,并进行各组织的病理学光镜检查。结果 :丹参能改善创伤性 AL I动物的血气 ,不同程度降低外周血 TNFα及 BAL F中 TNFα、C3a、C5 a和白蛋白含量 ,抑制肺、肝、心、肾组织中TNFα的蛋白表达 ,减轻内脏组织的损伤程度。结论 :丹参能减少组织及血浆 TNFα的生成及含量 ,降低TNFα等细胞因子介导的多器官组织损害 ,在一定程度上缓解 AL I和 MODS的发生、发展
Objective: To investigate the therapeutic effects and possible mechanisms of salvia miltiorrhiza on traumatic acute lung injury (AL I) and multiple organ dysfunction syndrome (MODS). METHODS: Using rabbit traumatic AL I model, experimental animals were randomly divided into normal control group (8 rats), traumatic AL group (8 rats), and Salvia miltiorrhiza treatment group (8 rats). Arterial blood gas and plasma of each group were performed. The content of tumor necrosis factor α (TNFα) in bronchoalveolar lavage fluid (BAL F) and the number of leukocytes in BAL F, and the contents of complement C3a, C5a, and albumin were determined. Immunohistochemistry combined with in situ quantitative analysis was used to detect the expression, distribution and relative content of TNFα protein in lung, liver, heart and kidney, and pathological light microscopy of each tissue was performed. Results: Salvia miltiorrhiza can improve the blood gas of traumatic AL I animals, reduce the content of TNFα, C3a, C5a and albumin in peripheral blood TNFα and BAL F, and inhibit the expression of TNFα protein in lung, liver, heart and kidney. The degree of damage to the visceral tissue. Conclusion :Salvia miltiorrhiza can reduce the production and content of TNFα in tissues and plasma and reduce the damage of multiple organ tissues mediated by cytokines such as TNFα. It can alleviate the occurrence and development of AL I and MODS to some extent.