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目的研究早期干预对宫内感染致脑损伤仔鼠脑组织S-100蛋白表达和神经行为学的影响。方法孕第18天的Wistar大鼠连续2d腹腔注射脂多糖(LPS),制备宫内感染模型,9g·L-1盐水组给予同剂量9g·L-1盐水。随机选取9g·L-1盐水组仔鼠30只(对照组)和LPS组仔鼠50只。LPS组仔鼠随机分为干预组(n=20)和非干预组(n=30)。出生第1天非干预组和对照组各取10只大鼠,断头处死,取其脑组织,应用免疫组织化学方法测定其S-100蛋白水平,并在出生第25天对3组大鼠S-100蛋白水平行免疫组织化学检测和神经行为学检测。结果LPS组母鼠胎盘病理检测见胎盘内血管充血、水肿,并见大量中性粒细胞浸润,9g·L-1盐水组母鼠胎盘病理检测未见明显炎症反应;非干预组1日龄仔鼠脑组织各部位S-100蛋白阳性染色与对照组仔鼠比较,均有明显增加(Pa<0.01);25日龄仔鼠中,非干预组S-100蛋白表达最强,对照组最弱,干预组介于两者之间,各组间两两比较差异有统计学意义(Pa<0.01);非干预组仔鼠在悬吊试验、姿势反射、肌张力、不自主运动、旷场试验中的得分明显低于干预组和对照组,干预组明显高于非干预组(Pa<0.05),但低于对照组(Pa<0.05);非干预组斜坡试验中所用时间最长,干预组所用时间明显短于非干预组(P<0.05),但所用时间仍长于对照组(P<0.05)。结论宫内感染可诱发新生大鼠脑损伤,S-100蛋白表达明显增多,早期干预可通过减少S-100蛋白表达,改善脑损伤仔鼠运动和行为异常。
Objective To investigate the effect of early intervention on the expression of S-100 protein and neuro-behavior in brain tissue of neonatal rats with intrauterine infection. Methods Wistar rats on the 18th day of pregnancy were injected intraperitoneally with lipopolysaccharide (LPS) for 2 days to prepare an intrauterine infection model. The rats in the 9g · L-1 saline group were given the same dose of 9g · L-1 saline. A total of 30 rats (control group), 9 g · L-1 saline group, and 50 LPS group were randomly selected. LPS group were randomly divided into intervention group (n = 20) and non-intervention group (n = 30). On the first day of life, 10 rats in the non-intervention group and the control group were sacrificed and decapitated. The brain tissues were harvested. The levels of S-100 protein were determined by immunohistochemistry. S-100 protein level immunohistochemistry and neurobehavioral testing. Results In placenta of placenta of rats in LPS group, placental congestion and edema were found in the placenta. A large amount of neutrophil infiltration was found in the placenta of rats in the LPS group. No significant inflammatory reaction was observed in the placenta of 9 g · L-1 saline group. Compared with the control group, the positive staining of S-100 protein in each part of rat brain tissue was significantly increased (Pa <0.01); in non-intervention group, the expression of S-100 protein was the strongest in control group (P <0.01). In non-intervention group, there was no significant difference in suspension test, postural reflex, muscle tension, involuntary movement and open-field test (P <0.05), but lower than that of the control group (Pa <0.05). The non-intervention group had the longest time in the slope test and the intervention group The time spent was significantly shorter than the non-intervention group (P <0.05), but the time spent was still longer than the control group (P <0.05). Conclusion Intrauterine infection can induce brain injury in neonatal rats and increase the expression of S-100 protein. Early intervention can reduce the expression of S-100 protein and improve the motor and behavioral abnormalities in neonatal rats.