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Aim:Botulinum neurotoxins (BoNT) abort the process of neurotransmitter re-lease at presynaptic motor nerve terminals,causing muscle paralysis.The abilityof botulinum toxin to produce its effect is dependent on the ability of the lightchain to cleave the SNARE proteins associated with transmitter release.Translo-cation of the light chain protease through the heavy chain-formed channel is apivotal step in the intoxication process.Toosendanin (TSN),a triterpenoid de-rivative extracted from a Chinese traditional medicine,has been demonstrated tobe an effective cure for experimental botulism.This study was designed to explorethe antibotulismic mechanisms of toosendanin.Methods:The inside-out single-channel recording patch-clamp technique was used to record the BoNT/A-in-duced currents in the presence and absence of TSN.Results:Channel formationwas delayed and the sizes of the channels were reduced in the TSN-treated PC12cell membrane.Conclusion:The antibotulismic effect of TSN might occur viainterference with toxin translocation.
Aim: Botulinum neurotoxins (BoNT) abort the process of neurotransmitter re-lease at presynaptic motor nerve terminals, causing muscle paralysis.The ability of botulinum toxin to produce its effect is dependent on the ability of the lightchain to cleave the SNARE proteins associated with transmitter release .Translo-cation of the light chain protease through the heavy chain-formed channel is apivotal step in the intoxication process.Toosendanin (TSN),a triterpenoid de-rivative extracted from a Chinese traditional medicine,has been demonstrated tobe an Effective cure for experimental botulism.This study was designed to explore the antibotulismic mechanisms of toosendanin.Methods:The inside-out single-channel recording patch-clamp technique was used to record the BoNT/A-in-duced currents in the presence and absence of TSN.Results: Channel formation was delayed and the sizes of the channels were reduced in the TSN-treated PC12cell membrane.Conclusion:The antibotulismic effect of TSN might occur v Iainterference with toxin translocation.