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目的探讨急性出血坏死性胰腺炎（ＡＮＰ）时中性粒细胞（ＰＭＮ）聚集于肺脏的机制。方法在ＡＮＰ并发急性肺损伤（ＡＬＩ）模型，采用免疫组化技术等方法，动态观察ＡＮＰ后大鼠肺脏实质细胞细胞间粘附分子１（ＩＣＡＭ１）、ＰＭＮ表面巨噬细胞分化抗原１（ＣＤ１１ｂ／ＣＤ１８）的表达改变和ＰＭＮ肺脏聚集的变化。结果ＡＮＰ的肺脏ＩＣＡＭ１表达逐渐增加，与ＰＭＮ肺脏聚集程度呈显著正相关，而ＰＭＮ表面ＣＤ１１ｂ／ＣＤ１８在ＡＮＰ后１小时即迅速表达至高峰，并持续２４小时。结论ＡＮＰ后，ＰＭＮ在肺脏的大量聚集是建立在ＰＭＮ表面ＣＤ１１ｂ／ＣＤ１８与肺实质细胞ＩＣＡＭ１高表达的基础之上，而ＩＣＡＭ１为这一粘附过程中的可变因素，是影响ＰＭＮ粘附并聚集于肺脏的主要因素。
Objective To investigate the mechanism of neutrophil (PMN) accumulation in the lung during acute hemorrhagic necrotizing pancreatitis (ANP). Methods Acute lung injury (ALI) model was induced by ANP. Immunohistochemistry and other methods were used to dynamically observe ICAM1 in lung parenchymal cells and differentiation of PMN on macrophages 1 (CD11b / CD18) expression changes and PMN lung aggregation changes. Results The expression of ICAM-1 in the lung of ANP increased gradually and positively correlated with the degree of pulmonary PMN accumulation. However, the expression of CD11b / CD18 on the surface of PMN rapidly peaked at 1 hour after ANP and lasted for 24 hours. Conclusions After ANP, the accumulation of PMN in the lungs is based on the high expression of CD11b / CD18 on the surface of PMN and ICAM-1 in lung parenchyma, and ICAM-1 is the variable factor in this process of adhesion The main factors that PMN adhere to and accumulate in the lungs.