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目的:研究小檗碱(Ber)对二硝基氟苯(DNFB)诱导的小鼠迟发型超敏反应(DTH)的影响,探讨其免疫抑制作用的机制。方法:BALB/c小鼠分为对照组、DTH组与Ber处理的DTH组。对照组以不含DNFB的溶剂处理,DTH组以5g/LDNFB腹部致敏、耳廓激发的方法建立模型,Ber处理的DTH组每天腹腔注射(i.p)Ber,连续7d,总剂量为30mg/kg体质量。激发后48h,取小鼠双耳称重了解Ber对炎症抑制率的影响。镜下观察耳廓局部组织的变化;以结晶紫法检查Ber对脾脏T淋巴细胞与细胞外基质(ECM)黏附作用的影响;以膜联蛋白V(AnnexinV)染色检查淋巴结细胞的凋亡率。结果:双耳称重的结果显示,Ber处理的DTH组与DTH组具有显著的差异(P<0.05),能明显抑制DNFB引起的炎症反应;耳廓局部组织学检测也表明,Ber能够抑制DNFB诱导的DTH,明显减少淋巴细胞的浸润。Ber处理的DTH组的T细胞与ECM的黏附能力明显降低(P<0.05)。Ber处理的DTH组与DTH组和对照组的细胞凋亡率均无明显差异(P>0.05)。结论:Ber对DNFB诱导的小鼠DTH具有明显的抑制作用,降低小鼠T细胞与ECM的黏附能力可能是其抑制DTH的机制之一。
Objective: To study the effect of berberine on delayed-type hypersensitivity (DTH) induced by dinitrofluorobenzene (DNFB) in mice and to explore the mechanism of immunosuppressive effect. Methods: BALB/c mice were divided into control group, DTH group and Ber treated DTH group. The control group was treated with DNFB-free solvent. The DTH group was modeled with 5 g/LDNFB abdominal sensitization and auricular challenge. The Ber-treated DTH group was injected intraperitoneally (ip) with Ber for 7 days, and the total dose was 30 mg/kg. Body mass. 48 hours after challenge, the ears were weighed and the effects of Ber on the inhibition rate of inflammation were determined. The changes in the local tissue of the auricle were observed under microscope; the effect of Ber on the adhesion of splenic T lymphocytes and extracellular matrix (ECM) was examined by crystal violet method; and the apoptosis rate of lymph node cells was examined by annexin V staining. RESULTS: The results of binaural weighing showed that there was a significant difference between Ber-treated DTH group and DTH group (P<0.05), which could significantly inhibit the inflammatory response induced by DNFB; local histological examination of the ear also showed that Ber could inhibit DNFB Induced DTH significantly reduced lymphocyte infiltration. The adhesion ability of T cells and ECM in Ber-treated DTH group was significantly lower (P<0.05). There was no significant difference in the apoptosis rate between the DTH group treated with Ber and the DTH group and the control group (P>0.05). Conclusion: Ber has a significant inhibitory effect on DTH induced by DNFB in mice, and decreasing the adhesion ability of mouse T cells to ECM may be one of the mechanisms of inhibition of DTH.