论文部分内容阅读
目的 :探讨胰岛素对培养的滋养细胞凋亡的影响及可能的机制。方法 :将培养的妊娠早期滋养层细胞 ,分为正常对照组(细胞 +培养液 )、H2 O2 组 (细胞 +培养液 +H2 O2 )和胰岛素 +H2 O2 组 (细胞 +H2 O2 +胰岛素 )。采用透射电镜观察及流式细胞术 ,观察H2 O2 诱导的细胞凋亡及胰岛素对H2 O2 诱导的细胞凋亡的抑制作用。并检测胰岛素对滋养细胞caspase 3的活性及Bcl 2蛋白表达的影响。结果 :H2 O2 可诱导培养的滋养细胞凋亡 ,透射电镜下可见特征性的细胞核改变。胰岛素可显著抑制H2 O2 诱导的细胞凋亡 ,流式细胞仪检测其凋亡率较H2 O2 组显著下降 (P <0 .0 1)。H2 O2 组滋养细胞中caspase 3的活性较对照组显著增高 (P <0 .0 1) ,而Bcl 2蛋白的表达则较对照组显著下降 (P <0 .0 1)。结论 :胰岛素可明显抑制H2 O2诱导的滋养细胞凋亡 ,其机制可能与降低caspase 3的活性和促进Bcl 2蛋白的表达有关
Objective: To investigate the effect of insulin on cultured trophoblast cells apoptosis and its possible mechanism. Methods: The trophoblast cells of early pregnancy were divided into normal control group (cell + culture medium), H2O2 group (cell + culture medium + H2 O2) and insulin + H2O2 group (cell + H2O2 + insulin). Transmission electron microscopy and flow cytometry were used to observe the H2O2-induced apoptosis and the inhibitory effect of insulin on H2O2-induced apoptosis. The effects of insulin on the activity of trophoblasts caspase 3 and the expression of Bcl 2 protein were also examined. Results H2O2 induced the apoptosis of trophoblast cells. The characteristic nuclear changes were observed under transmission electron microscope. Insulin can significantly inhibit H2O2-induced apoptosis, the apoptosis rate was significantly decreased by flow cytometry H2O2 group (P <0.01). The activity of caspase 3 in trophoblast cells in H2 O2 group was significantly higher than that in control group (P <0.01), while the expression of Bcl 2 protein was significantly decreased compared with control group (P <0.01). Conclusion: Insulin can significantly inhibit the apoptosis of trophoblast cells induced by H2O2. The mechanism may be related to the decrease of caspase 3 activity and the expression of Bcl2 protein