目的:探讨牛珀至宝微丸对内毒素休克大鼠脑神经型一氧化氮合酶(neuronalnitricoxide synthase,nNOS)表达的影响。方法:将SD大鼠随机分成正常对照组、模型组、牛珀至宝微丸组。模型组静 脉注射内毒素脂多糖(lipopolysaccharide,LPS)1.5mg/kg、腹腔注射D 氨基半乳糖(D galactosamine, D GalN)100mg/kg造成内毒素休克模型,牛珀至宝微丸组用药7d后再作以上处理。用免疫组织化学方法 检测各组nNOS在不同脑区的表达。结果:nNOS阳性细胞广泛分布于大脑皮质Ⅱ、Ⅲ、Ⅳ层,海马分子层, 齿状回多形层,脑干网状结构,小脑分子层、颗粒层和普尔基涅细胞层。在大脑皮质、海马、脑干及小脑各部 位,牛珀至宝微丸组的nNOS阳性细胞数略高于正常对照组,但明显低于模型组(P<0.05)。结论:牛珀至 宝微丸有部分下调内毒素休克所致广泛脑区nNOS过度表达的作用。 Objective: To investigate the effect of Niupo Zhibao Pellet on the expression of neuronal nitric oxide synthase (nNOS) in rats with endotoxin shock. Methods: SD rats were randomly divided into normal control group, model group and Niupo Zhibao Pellet group. The model group received intravenous injection of lipopolysaccharide (LPS) 1.5 mg/kg and intraperitoneal injection of D galactosamine (D GalN) 100 mg/kg to induce an endotoxin shock model. The Niupo Zhibao pellet group was administered 7 days later. Do the above processing. Immunohistochemistry was used to detect the expression of nNOS in different brain regions. RESULTS: nNOS-positive cells were widely distributed in the cerebral cortex II, III, and IV layers, the hippocampal molecular layer, the dentate gyri polymorph, the brain stem reticular formation, the cerebellar molecular layer, the granular layer, and the Purkinje cell layer. In the cerebral cortex, hippocampus, brainstem and cerebellum, the number of nNOS positive cells in Niupo Zhibao Pellet group was slightly higher than that in the normal control group, but was significantly lower than that in the model group (P<0.05). Conclusion: Niupozhibao Pills partially reduce the overexpression of nNOS in the extensive brain regions caused by endotoxin shock.