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氨苄青霉素(ABPC)为一广谱、耐酸抗生素,可口服从胃肠道吸收,大部分以原型从尿中排泄。对于半衰期比成人长的新生儿、儿童或肾功能不良病人和对各种制剂的生物等效利用度研究,测定尿中ABPC显得尤为重要。本法具有快速、灵敏、准确和专一性高等特点。色谱条件及方法:250mm×4.1mm id不锈钢柱(外套一保温柱),内装6.5μSynchropack RP-P.,流动相为pH4的0.01M醋酸钠溶液与甲醇混合液(9∶1),用前过滤脱气。流速1ml/min,检测波长225nm。ABPC标准液及头孢西丁内标液均用流动相新鲜配制,浓度都为0.1mg/ml。精密吸取ABPC
Ampicillin (ABPC) is a broad spectrum of acid-fast antibiotics that can be taken orally from the gastrointestinal tract and mostly excreted in urine as a prototype. It is particularly important to determine ABPC in urine for studies of the bioequivalence of various agents in neonates with longer half-life than adults, in children or in patients with impaired renal function. This law is fast, sensitive, accurate and specific high characteristic. Chromatographic conditions and methods: 250mm × 4.1mm id stainless steel column (coat a thermal column), containing 6.5μSynchropack RP-P, mobile phase pH 4 0.01M sodium acetate solution and methanol mixture (9: 1), pre-filtration Degassing. Flow rate 1ml / min, detection wavelength 225nm. ABPC standard solution and cefoxitin internal standard solution were freshly prepared with mobile phase, the concentration of 0.1mg / ml. Precision drawing ABPC