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目的探讨环境雌激素壬基酚(NP)暴露对雌性SD大鼠胸腺组织及胸腺淋巴细胞的损伤作用。方法将32只健康8～9周龄SPF级雌性SD大鼠按体重随机分为溶剂对照组(玉米油)和低(20 mg/kg)、中(80 mg/kg)、高(200 mg/kg)剂量NP组,每组8只,采用灌胃方式染毒,灌胃容量5 ml/kg,隔天染毒1次,连续60 d,观察胸腺组织病理学改变;采用流式细胞仪检测胸腺淋巴细胞内活性氧(ROS)、游离钙离子([Ca2+]i)浓度、线粒体膜电位(MMP)水平变化。结果溶剂对照组大鼠胸腺重量为(0.633±0.092)g,高剂量NP组为(0.514±0.084)g,差异有统计学意义(P<0.05),胸腺重量和染毒剂量呈负相关(r=-0.317,P<0.05);溶剂对照组与高剂量NP组大鼠胸腺淋巴细胞内ROS水平分别为(4.59±0.25)和(5.57±0.36),差异有统计学意义(P<0.05),胸腺淋巴细胞内ROS水平与NP浓度呈正相关(r=0.486,P<0.05);病理学检查可见,中、高剂量NP组胸腺皮质变薄。结论 NP对雌性大鼠胸腺及胸腺淋巴细胞具有一定毒性作用,并可引起免疫功能损伤。
Objective To investigate the effects of environmental estrogen nonylphenol (NP) exposure on thymus tissue and thymus lymphocytes in female SD rats. Methods 32 healthy female Sprague Dawley (SDF) rats aged 8-9 weeks were randomly divided into control group (corn oil) and low (20 mg / kg), medium (80 mg / kg) kg) in the NP group. Eight rats in each group were treated with intragastric administration of 5 ml / kg intragastrically and once a day for 60 consecutive days. Pathological changes of thymus were observed. Flow cytometry The levels of reactive oxygen species (ROS), free calcium ([Ca2 +] i) and mitochondrial membrane potential (MMP) in thymic lymphocytes were measured. Results The weight of thymus in the solvent control group was (0.633 ± 0.092) g and that of the high dose NP group was (0.514 ± 0.084) g, the difference was statistically significant (P <0.05) = -0.317, P <0.05). The levels of ROS in thymus lymphocytes of the solvent control group and the high dose NP group were (4.59 ± 0.25) and (5.57 ± 0.36), respectively, with significant difference (P <0.05) The level of ROS in thymus lymphocytes was positively correlated with NP concentration (r = 0.486, P <0.05). Thymic cortex was thinner in middle and high dose NP group. Conclusion NP has some toxic effects on thymus and thymus lymphocytes in female rats and can cause immune dysfunction.